SELEX identifies high-affinity RNA targets for chromatin-binding proteins PARP1 and MeCP2.

Recent discoveries showed that some chromatin-binding proteins also interact with RNA to regulate gene expression. Poly (ADP-ribose) polymerase 1 (PARP1) and methyl-CpG binding protein 2 (MeCP2) are two chromatin-associated, DNA-binding proteins that play central roles in gene expression, DNA damage response, and epigenetic regulation. Both proteins possess RNA-binding properties, but the mechanism by which PARP1 and MeCP2 recognize RNA-binding sites remains unclear.

Rehabilitating Soldiers for Load Carriage Tasks: An International Perspective.

Soldiers are likely to suffer an injury and require rehabilitation at some stage of their career. Load carriage, whilst a fundamental requirement, is also a source of injury risk. To optimize the rehabilitation of soldiers and prepare them for a full return to operational duty, load carriage requirements need to be considered throughout their rehabilitation pathway.

Medical data sharing and synthetic clinical data generation - maximizing biomedical resource utilization and minimizing participant re-identification risks.

The sensitive nature of electronic health records (EHR) and wearable data presents challenges in sharing biomedical resources while minimizing re-identification risks. This article introduces an end-to-end, titratable pipeline that generates privacy-preserving "digital twin" datasets from complex EHR and wearable-device records (Apple Watch data from 3029 participants) using DataSifter and Synthetic Data Vault (SDV) methods. Various obfuscation levels were applied (DataSifter: small, medium, large; SDV: CTGAN, Gaussian Copula) and benchmarked using utility (statistical fidelity, machine learning performance) and privacy (re-identification risk, detection likelihood) metrics.

Primary auditory cortex is necessary for the acquisition and expression of categorical behavior.

The primary auditory cortex (ACtx) extracts behaviorally relevant information from complex sounds and supports the categorization of continuous acoustic features into discrete perceptual or behavioral classes. Although ACtx is known to be necessary for certain sound-guided behaviors, how its contributions evolve during learning remains unclear. This evolving role may be shaped by the diversity of its excitatory output pathways, which include multiple classes of projection neurons that transmit sensory information to downstream targets.

Greater appeal and greater harm? Young adults' preference for menthol vs non-menthol very low nicotine cigarettes in a cross-over trial.

Introduction: The US Food and Drug Administration (FDA) proposed rulemaking to reduce cigarette nicotine content and ban menthol in cigarettes. Menthol is popular among young adults who smoke, though it is unclear how this group would respond if both policies were implemented.

Methods: 112 young adults who smoke menthol cigarettes completed three visits that varied by cigarette smoked (2021-2024): usual brand (UB) menthol, menthol very low nicotine cigarette (VLNC; 0.