Effective detection of staphylococcal infections in human bone tissue using combined raman microscopy and micro-computed tomography.

Bone infections caused by and are serious complications in orthopedic surgery. These infections commonly occur in joint replacements, fracture management, and bone grafting procedures. Rapid and accurate pathogen-specific diagnostic methods are urgently needed to support early clinical decisions.

The peptidoglycan-binding domain of a mycobacterial prophage-encoded LysinB reveals diverse approaches for domain conservation in hydrolases.

Mycobacteriophage-encoded LysinB enzymes target mycolyl ester linkages in mycolyl-arabinogalactan-peptidoglycan of mycobacterium hosts and generally exhibit a globular architecture. Here, we present the structural and functional characterization of a novel Mycobacterium fortuitum prophage-encoded modular LysinB (LysinB_MF), which contains the α/β hydrolase domain and a distinct peptidoglycan-binding domain (PGBD). The enzyme's active site features the conserved Ser-Asp-His catalytic triad common to esterases and forms a funnel-like topology.

Rosuvastatin-loaded injectable immunomodulatory hydrogel mitigates local immune response against transplanted stem cells and promotes heart repair in vivo.

In clinical trials allogeneic mesenchymal stem cells (MSCs) from young and healthy donors have shown promise to repair the heart following a heart attack. However, immune rejection of transplanted MSCs has prevented the clinical translation of stem cells-based therapies for cardiac patients. Therefore, strategies to improve survival of implanted stem cells in the heart would be of immense therapeutic value.

High-resolution liquid metal-based stretchable electronics enabled by colloidal self-assembly and microtransfer printing.

Liquid metal-based stretchable electronics offer high electrical performance and seamless integration with deformable systems but face challenges in achieving scalable, high-resolution patterning. In this work, we present a method for micropatterning liquid metal particle (LMP) films with feature sizes as small as 5 micrometers by integrating electrostatically enabled colloidal self-assembly and microtransfer printing. The resulting cold-welded LMP micropatterns exhibit exceptional electromechanical properties, high conductivity (2.

Unlocking the Techno-Functional and Biosafety Potential of a Novel Yeast Meyerozyma Guilliermondii YB1 as a Fermented Functional Food Starter: A Future Vision for Probiotics.

The present study investigates the techno-functional and biosafety potential of Meyerozyma guilliermondii YB1 (OQ832654) as a novel starter culture for functional foods. M. guilliermondii YB1 exhibited xylanase and phytase activity, with an activity index (AI) of 0.