Publications by authors named "Prakash Sivakumar"
Plants alternate between diploid sporophyte and haploid gametophyte generations. In mosses, which retain features of ancestral land plants, the gametophyte is dominant and has an independent existence. However, in flowering plants the gametophyte has undergone evolutionary reduction to just a few cells enclosed within the sporophyte.
View Article and Find Full Text PDFImproved validation of protein interactions using bicistronic BiFC (Bi2FC).
Physiol Mol Biol Plants
July 2024
Article Synopsis
- A new method for studying protein interactions called improved Bimolecular Fluorescence Complementation (BiFC) has been developed, focusing on better detection of protein expression levels.
- This method uses a bicistronic system that links the protein of interest with a fluorescent marker through a self-cleaving peptide, allowing for more accurate identification of interactions.
- Interestingly, the study also reveals that a previously identified non-interacting protein pair is actually interacting when observed in live cells, demonstrating the method's effectiveness.