Colchicine- and oryzalin-induced hexaploidy enhances nutrient levels and is a promising way to create new germplasms for Chinese chives.

Chinese chives reproduce primarily through apomixis, which greatly limits hybrid breeding efforts. Herein, germinated triploid Chinese chive seeds were used as the materials, and colchicine and oryzalin were applied to induce chromosome doubling. Observations of the growth habits and analysis of several secondary metabolites were compared between triploid and hexaploid Chinese chives.

Varied chromosome distribution behaviours during meiosis in triploid Chinese chives contribute to the formation of viable pollen.

Triploids play an important role in the polyploidization process and are considered a bridge between diploids and polyploids. To inform plant polyploidization research and polyploid breeding, it is important to explore chromosome behaviour during triploid pollen development, pollen fertility problems in triploids and the potential value of utilizing triploids. In this study, acetocarmine, carbol fuchsin and fluorescence staining methods were used to observe microsporogenesis and microspore development in fertile triploid Chinese chives.

Stomata variation in the process of polyploidization in Chinese chive (Allium tuberosum).

Background: Stomatal variation, including guard cell (GC) density, size and chloroplast number, is often used to differentiate polyploids from diploids. However, few works have focused on stomatal variation with respect to polyploidization, especially for consecutively different ploidy levels within a plant species. For example, Allium tuberosum, which is mainly a tetraploid (2n = 4x = 32), is also found at other ploidy levels which have not been widely studied yet.