Publications by authors named "Nina K Ramachandran"
Like many viruses, rotavirus (RV) dysregulates calcium homeostasis by elevating cytosolic calcium ([Ca]cyt) and decreasing endoplasmic reticulum (ER) stores. While an overall, monophasic increase in [Ca]cyt during RV infection has been shown, the nature of the RV-induced aberrant calcium signals and how they manifest over time at the single-cell level have not been characterized. Thus, we generated cell lines and human intestinal enteroids (HIEs) stably expressing cytosolic and/or ER-targeted genetically-encoded calcium indicators to characterize calcium signaling throughout RV infection by time-lapse imaging.
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- * Traditional small molecule fluorescent calcium dyes have limitations such as poor loading and retention in virus-infected cells, making it difficult to analyze calcium changes accurately.
- * Genetically-encoded calcium indicators (GECIs), including new variants that target different cellular compartments, have been developed to provide more reliable and long-term monitoring of calcium signals during virus infections.