Publications by authors named "Navrita Mathiah"
During the epithelial-mesenchymal transition driving mouse embryo gastrulation, cells divide more frequently at the primitive streak, and half of those divisions happen away from the apical pole. These observations suggest that non-apical mitoses might play a role in cell delamination. We aim to uncover and challenge the molecular determinants of mitosis position in different regions of the epiblast through computational modeling and pharmacological treatments of embryos and stem cell-based epiblast spheroids.
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- During gastrulation, a collection of epiblast cells forms the primitive streak, a critical area responsible for creating the mesoderm and endoderm layers.
- Live imaging of mouse embryos shows that cells in the posterior epiblast are more likely to form rosettes and undergo mitosis than cells in other areas of the epiblast.
- Non-apical mitosis is common in the primitive streak, which may help cells transition from an epithelial to a mesenchymal state necessary for further development.
Epithelial-mesenchymal transition (EMT) is often studied in pathological contexts, such as cancer or fibrosis. This chapter focuses on physiological EMT that allows the separation of germ layers during mouse embryo gastrulation. In order to record individual cells behavior with high spatial and temporal resolution live imaging as they undergo EMT, it is very helpful to label the cells of interest in a mosaic fashion so as to facilitate cell segmentation and quantitative image analysis.
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- - This study focuses on the role of Apoptosis-Inducing Factor (AIF) in mitochondrial function and cell death, highlighting its significance in severe pediatric mitochondrial diseases and cancer progression due to mutations affecting AIF.
- - Researchers created a new AIF-deficient mouse model to investigate the cellular and developmental consequences of AIF loss, using various molecular and cell biology methods to observe changes in cell behavior, metabolism, and overall phenotype.
- - Findings revealed that AIF deficiency disrupts mitochondrial electron transport, leading to ROS overproduction and metabolic reprogramming towards anaerobic glycolysis, with some cells showing resilience by reducing mitochondrial mass and escaping programmed cell death.
In mouse embryo gastrulation, epiblast cells delaminate at the primitive streak to form mesoderm and definitive endoderm, through an epithelial-mesenchymal transition. Mosaic expression of a membrane reporter in nascent mesoderm enabled recording cell shape and trajectory through live imaging. Upon leaving the streak, cells changed shape and extended protrusions of distinct size and abundance depending on the neighboring germ layer, as well as the region of the embryo.
View Article and Find Full Text PDFThe heart arises from distinct sources of cardiac progenitors that independently express Mesp1 during gastrulation. The precise number of Mesp1 progenitors that are specified during the early stage of gastrulation, and their clonal behavior during heart morphogenesis, is currently unknown. Here, we used clonal and mosaic tracing of Mesp1-expressing cells combined with quantitative biophysical analysis of the clonal data to define the number of cardiac progenitors and their mode of growth during heart development.
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