Comparative thoracic radiography in healthy and tuberculosis-positive sun bears ().

Early and accurate diagnosis of pulmonary tuberculosis (TB) is key to effective outbreak management, and in humans thoracic radiography is used extensively for screening purposes. In wildlife TB radiography is a relatively accessible diagnostic tool, particularly in under-resourced settings, however its use is limited by body size. Sun bears are susceptible to human-associated TB, and their small body size makes thoracic radiography feasible.

THUMPD3 regulates alternative splicing of ECM transcripts in human lung cancer cells and promotes proliferation and migration.

RNA-modifying enzymes have recently garnered considerable attention due to their relevance in cancer biology, identifying them as potential targets for novel therapeutic intervention. THUMPD3 was recently identified as an RNA methyltransferase catalysing N2-methylguanosine (m2G) within certain tRNAs. In this study, we unveil a novel role for THUMPD3 in lung cancer cells.

A blinded, placebo-controlled study on the clinical effects of vitamin E supplementation in dogs with osteoarthritis.

Background: Vitamin E has a positive effect in the management of osteoarthritis in humans, and in a previous study of dogs. It has been suggested to decrease C-reactive protein concentrations and liver enzyme activities in humans and animals.

Objective: To assess the effect of vitamin E supplementation on lameness, pain, pain medication requirement, clinical pathology variables, and quality of life in large-breed dogs with naturally occurring osteoarthritis.

Towards SINEUP-based therapeutics: Design of an synthesized SINEUP RNA.

SINEUPs are a novel class of natural and synthetic non-coding antisense RNA molecules able to increase the translation of a target mRNA. They present a modular organization comprising an unstructured antisense target-specific domain, which sets the specificity of each individual SINEUP, and a structured effector domain, which is responsible for the translation enhancement. In order to design a fully functional transcribed SINEUP for therapeutics applications, SINEUP RNAs were synthesized with a variety of chemical modifications and screened for their activity on endogenous target mRNA upon transfection.

Frequency, severity, and implications of shoulder pain in people with major upper limb amputation who use prostheses: Results of a National Study.

Background: People with upper limb amputation are potentially at increased risk of shoulder pain because they often perform compensatory movements to operate their prostheses and rely more heavily on their nonamputated limb for everyday activities.

Objective: To describe the frequency, severity, associated factors, and implications of shoulder pain in people with unilateral major upper limb amputation who use prostheses.

Design: Cross-sectional, observational design.

Small-molecule inhibition of METTL3 as a strategy against myeloid leukaemia.

N-methyladenosine (mA) is an abundant internal RNA modification that is catalysed predominantly by the METTL3-METTL14 methyltransferase complex. The mA methyltransferase METTL3 has been linked to the initiation and maintenance of acute myeloid leukaemia (AML), but the potential of therapeutic applications targeting this enzyme remains unknown. Here we present the identification and characterization of STM2457, a highly potent and selective first-in-class catalytic inhibitor of METTL3, and a crystal structure of STM2457 in complex with METTL3-METTL14.

Exploring newly qualified nurses' experiences of support and perceptions of peer support online: A qualitative study.

Aims And Objectives: To explore newly qualified nurses' support needs and their perceptions of online peer support.

Background: The experience of being a newly qualified nurse is stressful and isolating. Support from colleagues and peers can enhance perceptions of competence and confidence in newly qualified nurses, improve well-being and aid retention.

Magnetic resonance imaging in dogs with neuroangiostrongyliasis (rat lungworm disease).

The magnetic resonance imaging (MRI) appearance of the brain and spinal cord in humans with neuroangiostrongyliasis (NA) due to Angiostrongylus cantonensis infection has been well reported. Equivalent studies in animals are lacking. This case series describes clinical and MRI findings in 11 dogs with presumptively or definitively diagnosed NA.

Anconeus muscle injury in a juvenile greyhound.

Objective: To report anconeus muscle injury in a greyhound.

Animal: One 11-month-old female greyhound.

Study Design: Case report METHODS: The dog presented with a 6-week history of mild left foreleg lameness.

What is the nature of Mental Capacity Act training and how do health and social care practitioners change their practice post-training? A narrative review.

Aim And Objective: To identify training strategies and determine how registered health and social care practitioners change their practice after Mental Capacity Act training.

Design: Narrative literature review.

Data Sources: Seventeen databases were searched up to December 2019: CINAHL, Social Care Online, PubMed, Social Policy and Practice, Discover, MEDLINE, Science Direct, Ovid, 0of Science, British Nursing Index, DH-Data and King's Fund Library Catalogue.

Using technology to support the emotional and social well-being of nurses: A scoping review.

Aims: (a) To review the literature on the use of technology to offer emotional and social support to nurses; (b) to identify and evaluate gaps in the research; (c) to assess whether a systematic review would be valuable and (d) to make recommendations for future research.

Design: A robust methodological scoping framework was used as the basis for this review design. A manualized systematic approach to quality appraisal was implemented.

METTL1 Promotes let-7 MicroRNA Processing via m7G Methylation.

7-methylguanosine (m7G) is present at mRNA caps and at defined internal positions within tRNAs and rRNAs. However, its detection within low-abundance mRNAs and microRNAs (miRNAs) has been hampered by a lack of sensitive detection strategies. Here, we adapt a chemical reactivity assay to detect internal m7G in miRNAs.

Experiences of peer support for newly qualified nurses in a dedicated online group: Study protocol.

Aim: To explore experiences of online peer support for newly qualified nurses.

Design: Qualitative study using semi-structured interviews and thematic analysis.

Method: Phase one involves conducting focus groups with newly qualified nurses in the West Midlands area to refine the online peer support environment.

Using technology to support the social and emotional well-being of nurses: A scoping review protocol.

Aims: (a) To review the published literature on the use of technology to provide social or emotional support to nurses, (b) to evaluate and identify gaps in the research, (c) to assess whether a systematic review would be valuable, (d) to make recommendations for future developments.

Design: The protocol for the scoping review has been developed in accordance with recommendations from several methodological frameworks, as no standardized protocol currently exists for this purpose.

Methods: Our protocol incorporates information about the aims and objectives of the scoping review, inclusion criteria, search strategy, data extraction, quality appraisal, data synthesis, and plans for dissemination.

Scorer and modality agreement for the detection of intervertebral disc calcification in Dachshunds.

Background: The Dachshund is a chondrodystrophic breed of dog predisposed to premature degeneration and calcification, and subsequent herniation, of intervertebral discs (IVDs). This condition is heritable in Dachshunds and breeding candidates are screened for radiographically detectable intervertebral disc calcification (RDIDC), a feature of advanced disc degeneration and a prognostic factor for clinical disease. RDIDC scoring has been previously shown to be consistent within scorers; however, strong scorer effect (subjectivity) was also reported.

AORTIC ANEURYSM, DISSECTION, AND RUPTURE IN SIX BILE-FARMED BEARS.

Across China and Southeast Asia, over 17,000 bears are currently farmed for bile, predominantly for traditional Chinese medicines. Bears on farms in China are cage confined and undergo repeated daily bile extraction facilitated by surgically implanted catheters or gallbladder fistulas. Numerous health problems have been reported in bile-farmed bears including peritonitis, abdominal hernias, and extraction site abscessation.

1,2,4-Triazolo-[1,5-a]pyridine HIF Prolylhydroxylase Domain-1 (PHD-1) Inhibitors With a Novel Monodentate Binding Interaction.

Herein we describe the identification of 4-{[1,2,4]triazolo[1,5-a]pyridin-5-yl}benzonitrile-based inhibitors of the hypoxia-inducible factor prolylhydroxylase domain-1 (PHD-1) enzyme. These inhibitors were shown to possess a novel binding mode by X-ray crystallography, in which the triazolo N1 atom coordinates in a hitherto unreported monodentate interaction with the active site Fe ion, while the benzonitrile group accepts a hydrogen-bonding interaction from the side chain residue of Asn315. Further optimization led to potent PHD-1 inhibitors with good physicochemical and pharmacokinetic properties.

Precision of spinal radiographs as a screening test for intervertebral disc calcification in Dachshunds.

Among dog breeds, the Dachshund has the highest lifetime incidence of intervertebral disc disease (IVDD). Intervertebral disc (IVD) calcification is an indicator of severe degeneration that predisposes to disc herniation. IVDD is heritable in Dachshunds, and in some countries, breeding candidates are screened to reduce IVDD occurrence by selecting dogs according to their score of radiographically detectable intervertebral disc calcification (RDIDC) and excluding dogs with ≥5 RDIDCs from breeding.

Thyroid bed fine needle aspiration in patients after thyroidectomy--a useful follow-up tool with proposed diagnostic categories.

After thyroidectomy for primary thyroid malignancies, patients are closely monitored for recurrence or metastasis. Fine needle aspiration (FNA) has been used as the appropriate diagnostic modality for occult masses identified radiographically in the thyroid bed. In this study, we report our institutional experience with FNA of the thyroid bed and propose practical diagnostic categories.

The effect of manual lung inflation vs. spontaneous inspiration on the cardiac silhouette in anesthetized dogs.

The purpose of this study was to assess radiographic heart size in anesthetized dogs, comparing radiographs made with the patient breathing spontaneously to radiographs obtained using positive pressure manual lung inflation. The hypothesis was that manual inflation would cause reduction in the cardiac size. With dogs in right recumbency a radiograph was made at peak spontaneous inspiration followed immediately a radiograph made with application of positive pressure manual inflation of the thorax.

Use of contrast ultrasonography in the diagnosis of metastatic feline visceral haemangiosarcoma.

A 12-year-old cat was presented for investigation of weight loss and inappetence. Radiography and conventional grey-scale ultrasonography showed a large mid-body splenic mass. Contrast enhanced ultrasonography of the liver demonstrated a hypoechoic left lateral lobe nodular mass during the peak and late portal-phases of liver enhancement.