Publications by authors named "Nahyun Koo"
This study presents the development and validation of a fluorescence-based high-performance liquid chromatography (HPLC) method for the quantification of alectinib in rat plasma, with a focus on the application of Analytical Quality by Design (AQbD) to bioanalytical method development. Unlike conventional QbD applications, which primarily address synthetic formulations or instrumental settings, this study systematically applied AQbD principles to the complex environment of biological matrices. Critical method parameters, including the organic phase ratio, buffer concentration, and flow rate, were identified through Failure Mode and Effects Analysis, and optimized using a Box-Behnken design.
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- Ponatinib is a strong tyrosine kinase inhibitor used to treat chronic myeloid leukemia and specific types of acute lymphoblastic leukemia, and accurate measurement of its levels in plasma is vital for expanding its clinical use.
- Researchers developed a sensitive high-performance liquid chromatography (HPLC) method with fluorescence detection (FLD) to quantify ponatinib in rat plasma, employing a systematic optimization strategy for improved accuracy and efficiency.
- This new method boasts better sensitivity, easier sample preparation, and quicker results compared to previous techniques, facilitating further pharmacokinetic research and clinical applications for ponatinib.