Whole genome sequence data of NIBL1955 isolated from rice.

Whole-genome sequencing was performed on NIBL1955 isolated from rice. The genome consisted of one chromosome and three plasmids, with a total size of 1,958,539 bp. It contained 1,887 predicted protein-coding genes, 15 rRNA genes, and 56 tRNA genes.

Screening of Protein Tyrosine Phosphatase 1B Inhibitors from Actinomycete Extracts Using Recombinant .

Protein tyrosine phosphatase 1B (PTP1B) removes phosphate groups from phosphorylated tyrosine proteins in human cells, particularly in the insulin and leptin signaling pathways. It is a key drug target for ailments such as type 2 diabetes and obesity. However, there is a lack of highly specific PTP1B inhibitor drugs.

Draft genome sequence data of NIYL22663.

NIYL22663, which was isolated from soil, is known for its enhanced production of organic acids and flavor compounds, making it valuable for fermentation and biotechnology applications. Despite these promising traits, its whole genome sequences remain underexplored. In this study, its whole genome was sequenced using the PacBio Sequel II and Illumina platforms.

Isolation of xylanase-producing yeast from chrysanthemum.

Yeast strains showing xylanase activity were explored from various natural sources, such as flowers and soil. A yeast strain showing high xylanase activity on the Remazol Brilliant Blue-xylan plate was isolated from the flower of yellow chrysanthemum and identified as through analysis of the nucleotide sequence of the internal transcribed spacer region of 18S ribosomal RNA. MBY/L1662 exhibited higher tolerance to fermentation inhibitors than the control strain.

Distillation method influences flavor characteristics of .

The impact of the distillation method on the flavor characteristics of traditional was investigated. Partial least squares-discrimination analysis (PLS-DA) was performed based on the volatile compounds detected by an electronic nose. This analysis revealed distinct flavor profiles when grouped by samples, with ethyl acetate and isoamyl alcohol showing high variable importance in the projection (VIP) scores.

Flavor patterns of beer fermented by .

Unlabelled: , formerly known as , is the yeast that produces ethanol and lactic acid in sour beer fermentation. The three strains of were isolated in this study, and their performances as starters for sour beer fermentation were evaluated All isolates exhibited comparable performances to the control in producing ethanol, acetic acid, and lactic acid. Electronic nose and gas chromatography/mass spectrometry analysis revealed that flavor patterns of beer varied notably according to the strains used.

Isolation of a Potential Probiotic and Evaluation of Its Exopolysaccharide for Antioxidant and α-Glucosidase Inhibitory Activities.

The probiotic properties of ten lactic acid bacteria and antioxidant and α-glucosidase inhibitory activities of the exopolysaccharide (EPS) of the selected strain were investigated in this study. L010 was one of the most active strains across all the in tests. The cell-free supernatant (50 g/l) of L010 showed high levels of both α-glucosidase inhibitory activity (98.

Draft genome sequence data of multi-stress tolerant yeast KCTC27753 isolated from nuruk.

The strain KCTC27753, isolated from nuruk, is a multi-stress tolerant yeast which grows at 46 °C temperature and pH 3.0. This strain can withstand fermentation inhibitors, such as furfural and phenolic compounds released from biomass.

Draft genome sequence data of the starch-utilizing yeast MBY1320 isolated from Nuruk.

MBY1320 was isolated from Nuruk, a traditional Korean starter for makgeolli (rice wine) production. This isolate has previously been reported to exhibit thermotolerance and starch-degrading activities. In this data description, we present the draft genome sequence of MBY1320.

Isolation of acid tolerant lactic acid bacteria and evaluation of α-glucosidase inhibitory activity.

In this study, lactic acid bacteria strains (LABs) were isolated from Korean traditional fermented food and examined as potential probiotics using in vitro methods. Ten LAB strains survived in de Man, Rogosa and Sharpe broth adjusted to pH 2.5 were tested for resistance to acidic conditions and bile, antimicrobial activity, and α-glucosidase inhibitory activity.

Complete genome sequence data of MBEL1397 isolated from kimchi.

MBEL1397(=KCTC14037BP) was isolated from kimchi, a traditional Korean fermented food, in Gangwon province, Republic of Korea. MBEL1397 is an acid-tolerant strain with antimicrobial activity and α-glucosidase inhibitory activity, which might be preliminary indications of its probiotic properties. Complete genome sequencing of MBEL1397 was performed using the PacBio RSII platform.

Complete genome sequence data of a broad-spectrum antipathogen, KCTC 18343P, isolated from Korean traditional rice wine.

KCTC18343P(=MBE1283) isolated from , Korean traditional rice wine, strongly inhibits the growth of food and plant pathogens. A complete genome sequence of KCTC18343P is presented in this report. The genome is 3,979,925 bp in size and harbors 3856 genes.

Changes in antioxidant activities and volatile compounds of mixed berry juice through fermentation by lactic acid bacteria.

The objectives of this study were to analyze antioxidant activities and identify volatile compounds in mixed berry juice after fermentation by lactic acid bacteria (LAB). Antioxidant activity of the mixed berry juice increased significantly from 209.57±2.

Isolation of the Inositol Phosphoceramide Synthase Gene (AUR1) from Stress-Tolerant Yeast Pichia kudriavzevii.

This study is the first report of the entire nucleotide sequence of an inositol phosphoceramide synthase gene from the stress-tolerant yeast Pichia kudriavzevii (PkAUR1). Sequence analysis revealed an open reading frame that spans 1,443 bp and encodes a 480-amino-acid-residue protein with the highest sequence similarity (41.7%) to Aur1 from Spathaspora passalidarum.

Optimization of Expression Conditions Enhances Production of Sepiapterin, a Precursor for Tetrahydrobiopterin Biosynthesis, in Recombinant Escherichia coli.

Sepiapterin is a precursor for the synthesis of tetrahydrobiopterin (BH4), which is a wellknown cofactor for aromatic amino acid hydroxylation and nitric oxide synthesis in higher mammals. In this study, a recombinant Escherichia coli BL21(DE3) strain harboring cyanobacterial guanosine 5'-triphosphate cyclohydrolase 1 (GCH1) and human 6- pyruvoyltetrahydropterin synthase (PTPS) genes was constructed to produce sepiapterin. The optimum conditions for T7 promoter-driven expression of GCH1 and PTPS were 30°C and 0.

Isolation of the Phosphoribosyl Anthranilate Isomerase Gene (TRP1) from Starch-Utilizing Yeast Saccharomycopsis fibuligera.

The nucleotide sequence of the TRP1 gene encoding phosphoribosyl anthranilate isomerase in yeast Saccharomycopsis fibuligera was determined by degenerate polymerase chain reaction and genome walking. Sequence analysis revealed the presence of an uninterrupted open-reading frame of 759 bp, including the stop codon, encoding a 252 amino acid residue. The deduced amino acid sequence of Trp1 in S.

Cysteine Protease Profiles of the Medicinal Plant Calotropis procera R. Br. revealed by de novo transcriptome analysis.

Calotropis procera R. Br., a traditional medicinal plant in India, is a promising source of commercial proteases, because the cysteine proteases from the plant exhibit high thermo-stability, broad pH optima, and plasma-clotting activity.

Cloning of the transketolase gene from erythritol-producing yeast Candida magnoliae.

The entire nucleotide sequence of the TKL1 gene encoding transketolase (TKL) in an erythritolproducing yeast of Candida magnoliae was determined by degenerate polymerase chain reaction and genome walking. Sequence analysis revealed an open reading frame of C. magnoliae TKL1 (CmTKL1) that spans 2,088 bp and encodes 696 amino acids, sharing 61.

Enhanced production of ε-caprolactone by coexpression of bacterial hemoglobin gene in recombinant Escherichia coli expressing cyclohexanone monooxygenase gene.

Baeyer-Villiger (BV) oxidation of cyclohexanone to epsilon-caprolactone in a microbial system expressing cyclohexanone monooxygenase (CHMO) can be influenced by not only the efficient regeneration of NADPH but also a sufficient supply of oxygen. In this study, the bacterial hemoglobin gene from Vitreoscilla stercoraria (vhb) was introduced into the recombinant Escherichia coli expressing CHMO to investigate the effects of an oxygen-carrying protein on microbial BV oxidation of cyclohexanone. Coexpression of Vhb allowed the recombinant E.

Overexpression, crystallization and preliminary X-ray crystallographic analysis of a putative xylose isomerase from Bacteroides thetaiotaomicron.

Bacteroides thetaiotaomicron BT0793, a putative xylose isomerase, was overexpressed in Escherichia coli, purified and crystallized using polyethylene glycol monomethyl ether 550 as the precipitant. X-ray diffraction data were collected to 2.10 Å resolution at 100 K using synchrotron X-rays.