Publications by authors named "Muhammad Fakhar-I-Adil"
In vitro maturation (IVM) of oocytes retrieved from ovum pick-up (OPU) or ovarian tissue (OT) is a standard approach for patients with specific conditions where prior hormonal stimulation is contraindicated. However, the developmental competence of oocytes matured in vitro is still inferior to that of oocytes matured in vivo. Capacitation IVM (CAPA-IVM) includes an extra step of pre-maturation culture (PMC) with c-type natriuretic peptide (CNP) as a meiotic arrestor to better synchronize cytoplasmic and nuclear maturity in oocytes by allowing the cytoplasm additional time to acquire essential components critical for optimal competency.
View Article and Find Full Text PDFPronuclear transfer rescues poor embryo development of -grown secondary mouse follicles.
Antonia Christodoulaki
,
Haitang He
,
Min Zhou
,
Chloë De Roo
,
Machteld Baetens
,
Muhammad Fakhar-I-Adil
Hum Reprod Open
February 2024
Article Synopsis
- The study investigates whether pronuclear transfer (PNT) can enhance embryo development in mouse oocytes that have been grown in vitro (IVG) but suffer from cytoplasmic deficiencies.
- Results show that using PNT with matured cytoplasm significantly improved the development of IVG oocytes, producing live and fertile offspring, suggesting a potential method to address fertility issues.
- Conducted from September 2020 to February 2022, this research involved 120 B6D2 mice and aimed to establish effective techniques for culture and maturation of ovarian follicles while comparing IVG oocytes to those grown in vivo.