Effect of temperature on the structure of α-l-fucosidase from : implications from molecular dynamics simulation.

Human milk oligosaccharides, like 2'-fucosyllactose, have beneficial effects on newborn health, and they can be obtained by enzymatic synthesis with α-l-fucosidases. In this work, the impact of temperature on the α-l-fucosidase from (TmαFuc) structure was evaluated using molecular dynamics simulation (MD). The TmαFuc was found stable in a temperature range of 333-368 K since no differences in the RMSD, RMSF, H-bonds, solvent accessible surface area, radius of gyration, salt bridges and native contacts () values were observed.

Improvement of Fucosylated Oligosaccharides Synthesis by α-L-Fucosidase from Thermotoga maritima in Water-Organic Cosolvent Reaction System.

The effects of water activity (a), pH, and temperature on transglycosylation activity of α-L-fucosidase from Thermotoga maritima in the synthesis of fucosylated oligosaccharides were evaluated using different water-organic cosolvent reaction systems. The optimum conditions of transglycosylation reaction were the pH range between 7 and 10 and temperature 90-95 °C. The addition of organic cosolvent decreased α-L-fucosidase transglycosylation activity in the following order: acetone > dimethyl sulfoxide (DMSO) > acetonitrile (0.