Publications by authors named "Mohd Afaque Ansari"
The field of healthcare diagnostics is navigating complex challenges driven by evolving patient demographics and the rapid advancement of new technologies worldwide. In response to these challenges, these biosensors offer distinctive advantages over traditional diagnostic methods, such as cost-effectiveness, enhanced specificity, and adaptability, making their integration with point-of-care (POC) platforms more feasible. In recent years, aptasensors have significantly evolved in diagnostic capabilities through the integration of emerging technologies such as microfluidics, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) systems, wearable devices, and machine learning (ML), driving progress in precision medicine and global healthcare solutions.
View Article and Find Full Text PDFIn this study, a nanocomposite was synthesized by incorporating graphitic carbon nanosheets, carboxyl-functionalized multiwalled carbon nanotubes, and zirconium oxide nanoparticles. The resulting nanocomposite was utilized for the modification of a glassy carbon electrode. Subsequently, matrix metalloproteinase aptamer (Apt) was immobilized onto the electrode surface through the application of ethyl-3-(3-(dimethylamino)propyl)carbodiimide hydrochloride--hydroxysuccinimide (EDC-NHS) chemistry.
View Article and Find Full Text PDFFor early detection and diagnosis of cancer, it is essential to develop an electrochemical biosensor that is quick, accurate, and sensitive. Here, we use gold nanorod (AuNR) and gold nanowire (AuNW) nanocomposites (AuNR/AuNW/CS) as electrode modifiers on a glassy carbon electrode (GCE) to construct a sensitive label-free electrochemical aptasensor to detect ApoA1. The thiolated ApoA1-specific aptamers were immobilized onto the modified electrode surface through self-assembled monolayers.
View Article and Find Full Text PDFArticle Synopsis
- New Delhi metallo-beta-lactamase (NDM-1) is a type of enzyme that can inactivate most beta-lactam antibiotics, including carbapenems, except for aztreonam, by breaking down their molecular structure.
- The study focuses on how various carbapenems, including Imipenem and Meropenem, interact with the NDM-1 enzyme through a process called molecular docking.
- Researchers used Autodock4.2 software to analyze these interactions, aiming to understand the relationship between NDM-1 and different carbapenem antibiotics.
Article Synopsis
- New Delhi metallo-beta-lactamase (NDM-1) is an enzyme that can break down nearly all beta-lactam antibiotics except aztreonam, using zinc ions for activity.
- The study investigates how NDM-1 interacts with various carbapenem antibiotics through molecular docking techniques.
- Autodock4.2 software was used to analyze the interactions between NDM-1 and multiple carbapenems individually, helping to understand the effectiveness of these drugs against the enzyme.
Background: In osteosarcoma tissue, both MMP-2 and MMP-9 are over expressed compared to their expression in non-affected stromal tissue. Hence, gelatinases are attractive targets for anti-osteosarcoma drugs.
Objective: To study the inhibitory activity of compounds isolated from Ageratum houstonianum against MMP-2 and MMP-9 by in-silico approach.