Phage Therapy: A Targeted Solution for Brucellosis.

Brucellosis is a widespread zoonotic bacterial infection that affects over 500,000 people annually, with significant economic losses in the livestock industry, particularly in endemic regions such as the Middle East, Asia, and parts of Africa and Latin America. Despite standard antibiotic treatments, relapse rates remain as high as 10%-15%, highlighting the limitations of current therapeutic options. Phage therapy, which uses bacteriophages to selectively target and lyse Brucella spp.

Evaluation of the Antibacterial and Anti-Biofilm Activity of Chitosan-Arginine Nanoparticles and Sodium Fluoride against .

Dental caries is among the most prevalent chronic diseases. It arises from bacterial biofilm formation on tooth surfaces due to metabolic activity. (.

Effect of photodynamic therapy with imipenem and gentamicin-derived carbon dots on the expression of quorum sensing and virulence genes in Pseudomonas aeruginosa.

Background: The capability of to cause various severe infections is linked to the production of various virulence factors, regulated by quorum sensing. The objective of this study was to assess the effect of sub-lethal doses of antimicrobial photodynamic inactivation (sAPDI), employing carbon dots (CDs) derived from gentamicin and imipenem, on the expression of genes in two key quorum sensing systems (the rhl and las systems) and on the genes responsible for producing elastase (), protease (), rhamnolipid (), and pyocyanin () in .

Methods: CDsGEN-NH, and CDsIMP-NH were synthesized from gentamicin and imipenem precursors through the hydrothermal method.

Exploring fluoroquinolone resistance mechanisms and the effects of carbonyl cyanide 3-chlorophenylhydrazone (CCCP) in .

Objectives: This study aims to investigate the prevalence and mechanisms of fluoroquinolone resistance in strains isolated from hospitals in Hamadan, west of Iran. It investigates the role of specific resistance genes and mutations in contributing to this resistance. In addition, the effects of carbonyl cyanide 3-chlorophenylhydrazone (CCCP) on the susceptibility of to fluoroquinolones will be evaluated to identify potential strategies to combat this growing problem.

Biofilm inhibition of multidrug-resistant Pseudomonas aeruginosa using green-synthesized silver nanoparticles and colistin.

We aimed to investigate the synergistic effects of colistin and green-synthesized silver nanoparticles on the biofilm formation and expression of Quorum Sensing regulated and related genes in clinical isolates of P. aeruginosa. Ten clinical P.

Gut microbiomes and treatment-resistant ulcerative colitis: a case-control study using qPCR.

Background: The gut microbiome has been identified as a pivotal factor in ulcerative colitis (UC), given its role as the main reservoir of microbes in the body. This community of microorganisms, present in variable concentrations in the digestive tract, makes a wide range of beneficial roles for the host. However, the role of the gut microbiome in patients with refractory UC is still significant, so this study aimed to further investigate the role of these bacteria in patients with refractory UC.

Improvement of the Antimicrobial and Antibiofilm Activity of AH Plus Sealer with Chitosan Nanoparticles and Arginine against Enterococcus faecalis and Candida albicans.

The success of endodontic treatments can be significantly impaired by persistent microbial pathogens, especially (. ) and (. ).

The effect of photodynamic therapy using gentamicin and imipenem-derived carbon dots on Pseudomonas aeruginosa isolates.

The increasing prevalence of antibiotic resistance poses a significant threat to the ability of healthcare systems to manage Pseudomonas aeruginosa infections, highlighting the need for innovative therapeutic strategies. The aim of this study was to evaluate the effects of photodynamic therapy (PDT) using carbon dots (CDs) derived from gentamicin and imipenem against P. aeruginosa isolates.

Investigating the expression of anti/pro-inflammatory cytokines in the pathogenesis and treatment of ulcerative colitis and its association with serum level of vitamin D.

Ulcerative colitis is an idiopathic gastrointestinal disease described by chronic inflammation of the digestive system. Cytokines may be responsible for immunopathogenesis, mucosal and tissue damage, and even treatment response. In addition to its role in calcium and phosphorus homeostasis and bone health, vitamin D is an immunomodulatory and anti-inflammatory agent.

Analysis of phylogroups, biofilm formation, virulence factors, antibiotic resistance and molecular typing of uropathogenic Escherichia coli strains isolated from patients with recurrent and non-recurrent urinary tract infections.

Background: Uropathogenic Escherichia coli (UPEC) is the predominant cause of urinary tract infections (UTIs), and the recurrence of these infections poses significant treatment challenges.

Objective: This study aimed to compare the phylogroups, biofilm formation, virulence factors, and antibiotic resistance of UPEC strains in patients with recurrent versus non-recurrent UTIs in Hamadan City, Western Iran.

Materials And Methods: A total of 110 E.

The role of vitamin D in treated and refractory ulcerative colitis patients: a case-control study.

Background: Ulcerative colitis is a form of chronic inflammatory bowel disease (IBD) marked by ongoing inflammation of the mucosal lining that extends from the rectum to the upper part of the colon. Vitamin D regulates immune responses in several autoimmune and inflammatory diseases, including ulcerative colitis. Therefore, the study aims to investigate the role of vitamin D in the pathogenesis and treatment of ulcerative colitis.

The antimicrobial and antibiofilm effects of gentamicin, imipenem, and fucoidan combinations against dual-species biofilms of Staphylococcus aureus and Acinetobacter baumannii isolated from diabetic foot ulcers.

Introduction: Diabetes mellitus is a chronic metabolic disorder characterized by persistent hyperglycemia due to impaired insulin production or utilization, leading to severe health complications. Diabetic foot ulcers (DFUs) represent a major complication, often exacerbated by polymicrobial infections involving Staphylococcus aureus and Acinetobacter baumannii. These pathogens, notorious for their resistance to antibiotics, complicate treatment efforts, especially due to biofilm formation, which enhances bacterial survival and resistance.

Antibacterial and antibiofilm activity of silver nanoparticles stabilized with C-phycocyanin against drug-resistant and .

Background: Biofilms are bacterial communities that can protect them against external factors, including antibiotics. In this study, silver nanoparticles (AgNPs) were formed by modifying AgNPs with C-phycocyanin (Ag-Pc) to inhibit the growth of carbapenem-resistant (CR ) and methicillin-resistant (MRSA) and destroy biofilm of these bacteria.

Methods: The AgNPs were prepared with the green synthesis method, and Pc was used to stabilize the AgNPs.

Investigating the Prevalence of Bacterial Infections in Patients with Coronavirus Disease 2019 Hospitalized in Intensive Care Unit and Determining their Antibiotic Resistance Patterns.

Background: COVID-19 patient hospitalization, particularly in intensive care units, exposes them to bacterial and fungi co-infections, which can have very serious consequences, including increased mortality. In addition, antibiotic resistance among pathogens is a hidden threat behind COVID-19.

Methods: In the period from 2020 September to 2021 August, bacterial isolates from COVID- 19 patients admitted to the ICU of Sina Hospital in Hamadan, Iran, were collected and identified based on standard biochemical tests.

A single-center analysis of clonal transmission of carbapenem-resistant Acinetobacter baumannii among intensive care unit patients during the COVID-19 pandemic.

Carbapenem-Resistant Acinetobacter baumannii (CRAB) outbreak in intensive care units (ICUs) is a significant problem for healthcare facilities. In this study, we aimed to investigate the occurrence of CRAB isolates among ICU-admitted patients during the three waves of the COVID-19 pandemic in Iran using Multiple-Locus Variable Number Tandem-Repeat Analysis (MLVA). We obtained 50 (A) baumannii isolates from tracheal aspirate and blood culture samples.

Effect of essential oil on A efflux pump gene expression and biofilm formation in ciprofloxacin-resistant strains.

Background And Objectives: Today, medicinal plants and their derivatives are considered to reduce the prevalence of antibiotic resistance. The aim of this study was to investigate the effect of essential oil on A efflux pump gene expression and biofilm formation in ciprofloxacin-resistant strains.

Materials And Methods: A total of 50 clinical strains of resistant to ciprofloxacin were studied.

Characterization of recombinant phytase of Klebsiella sp. and the influence of novel 3-phytase on mineral solubility in broiler diets under an in vitro digestion assay.

Phytate (inositol hexaphosphate) is the major storage form of phosphorus (P) in nature, and phytases catalyze the hydrolysis of P from phytate and the formation of inositol phosphate isomers. In this study, a bacterium that produces phytase was isolated in a phytase screening medium. The bacterium was identified as Klebsiella sp.

Staphopain mediated virulence and antibiotic resistance alteration in co-infection of Staphylococcus aureus and Pseudomonas aeruginosa: an animal model.

Polymicrobial communities lead to worsen the wound infections, due to mixed biofilms, increased antibiotic resistance, and altered virulence production. Promising approaches, including enzymes, may overcome the complicated condition of polymicrobial infections. Therefore, this study aimed to investigate Staphopain A-mediated virulence and resistance alteration in an animal model of Staphylococcus aureus and Pseudomonas aeruginosa co-infection.

Synergistic effects of nano curcumin mediated photodynamic inactivation and nano-silver@colistin against Pseudomonas aeruginosa biofilms.

Background: Patients with burn injuries colonized by multidrug-resistant Pseudomonas aeruginosa face increased mortality risk. The efficacy of colistin, a last-resort treatment, is declining as resistance levels rise. P.

The healing effect of Pseudomonas Quinolone Signal (PQS) with co-infection of Staphylococcus aureus and Pseudomonas aeruginosa: A preclinical animal co-infection model.

Background: Because of the rise in antibiotic resistance and the control of pathogenicity, polymicrobial bacterial biofilms exacerbate wound infections. Since bacterial quorum sensing (QS) signals can dysregulate biofilm development, they are interesting therapeutic treatments. In this study, Pseudomonas Quinolone Signal (PQS) was used to treat an animal model of a wound that had both Staphylococcus aureus and Pseudomonas aeruginosa co-infection.