Review of Toxoplasmosis: What We Still Need to Do.

is responsible for the disease toxoplasmosis and has the broadest host range among apicomplexan parasites, as it infects virtually all warm-blooded vertebrates. Toxoplasmosis is a zoonotic and emerging public health concern with considerable morbidity and mortality, especially in the developing world, affecting approximately one-third of the world's human population. Clinical presentation varies among species, and the infection establishes lifelong chronicity in hosts.

Targeted Gene Knock-Out of in Fetal Fibroblasts Using CRISPR-Cas9: Implications for Cat Allergies.

is the most important allergen secreted by cats, which can trigger asthma in sensitive individuals. Our objective was to knock-out the gene in the fetal fibroblasts of cats through CRISPR-Cas9 technology with two sgRNAs and to determine the impact of such mutations on the antigenicity of the protein. DNA samples from 38 domestic cats were collected and amplified by PCR to obtain the complete sequence of the gene.