Publications by authors named "Milena D Vukic"

The emergence of antimicrobial resistance and the increasing demand for a healthier lifestyle have set new goals for science and industry. In the search for new, more effective, and environmentally friendly antimicrobial agents, special attention is being paid to natural resources. In this regard, essential oils derived from plants, which are widely used in the cosmetic, food, and pharmaceutical industries, are one of the solutions.

View Article and Find Full Text PDF

is mostly grown for its usage in the food, medical, and perfume industries, while it is also used as an attractive plant in parks, gardens, and homes. The use of essential oil may yield new results in relation to the antimicrobial activity of essential oils and their use mainly in extending the shelf life of foods. This study investigates the chemical composition and antimicrobial properties of essential oil (RDEO) using gas chromatography-mass spectrometry (GC-MS) and various bioassays to explore its potential applications in food preservation and microorganism growth control.

View Article and Find Full Text PDF

, commonly known as star anise, represents one of the notable botanical species and is recognized for its rich reservoir of diverse bioactive compounds. Beyond its culinary application as a spice, this plant has been extensively utilized in traditional medicine. Given the contemporary emphasis on incorporating natural resources into food production, particularly essential oils, to enhance sensory attributes and extend shelf life, our study seeks to elucidate the chemical composition and evaluate the antibacterial (, ) and insecticidal properties of essential oil (IVEO).

View Article and Find Full Text PDF

New goals for industry and science have led to increased awareness of food safety and healthier living in the modern era. Here, one of the challenges in food quality assurance is the presence of pathogenic microorganisms. As planktonic cells can form biofilms and go into a sessile state, microorganisms are now more resistant to broad-spectrum antibiotics.

View Article and Find Full Text PDF
Article Synopsis
  • Pharmacological agents with antioxidant properties play a crucial role in preventing and treating health issues linked to oxidative stress, such as cancer.
  • A study assessed 14 new compounds called N-pyrocatechoyl and N-pyrogalloyl hydrazones (N-PYRs), revealing their strong ability to neutralize harmful free radicals and their beneficial antioxidant pathways.
  • Further analysis showed that four of these compounds selectively targeted cancer cells while also demonstrating antibacterial effects, indicating their potential as effective antioxidant agents with dual therapeutic applications.
View Article and Find Full Text PDF

Background: Shikonin is a naturally occurring naphthoquinone found in the roots of several genera of the Boraginaceae family, widely known for its numerous biological activities, such as antiinflammatory, antioxidant, antimicrobial and anticancer. In this study, the antitumor effect of six naphthoquinones isolated from the roots of Onosma visianii was evaluated using two cell lines, mouse melanoma B16 and highly aggressive rat glioma cell line C6.

Methods And Results: All examined shikonins dose-dependently decreased the viability of tested cells, with compounds 5 and 6 being the most potent ones and hence subjected to further analysis.

View Article and Find Full Text PDF

With the growing issues of food spoilage, microbial resistance, and high mortality caused by cancer, the aim of this study was to evaluate essential oil (TZEO) as a potential solution for these challenges. Here, we first performed GC/MS analysis which showed that the tested TZEO belongs to the linalool chemotype since the abundance of linalool was found to be 38.0%.

View Article and Find Full Text PDF
Article Synopsis
  • The study analyzed the chemical composition and various biological activities of SSEO, highlighting major components like linalool acetate and linalool, with a focus on their antioxidant, antimicrobial, antibiofilm, and insecticidal properties.
  • Antioxidant activity was relatively low, with SSEO showing limited capacity to neutralize DPPH and ABTS radicals.
  • The antimicrobial tests showed moderate effectiveness, with ()-caryophyllene displaying the strongest antimicrobial activity and SSEO proving more effective in vapor form against biofilms and as an insecticide.
View Article and Find Full Text PDF

The aim of this study was to evaluate the antioxidant, antibiofilm, antimicrobial (in situ and in vitro), insecticidal, and antiproliferative activity of essential oil (CSEO) obtained from the plant leaf. The identification of the constituents contained in CSEO was also intended by using GC and GC/MS analysis. The chemical composition revealed that this sample was dominated by monoterpene hydrocarbons α-pinene, and δ-3-carene.

View Article and Find Full Text PDF
Article Synopsis
  • This study focused on essential oil (EGEO), evaluating its chemical composition, antimicrobial, antioxidant, antibiofilm, and insecticidal activities.
  • Key components of EGEO were identified, with 1,8-cineole being the most abundant at 63.1%.
  • Results showed strong antimicrobial effects, particularly in vapor form, with significant insecticidal activity at varying concentrations and notable antioxidant capacity.
View Article and Find Full Text PDF
Article Synopsis
  • * The essential oils exhibit significant antimicrobial effects, outperforming commercial antibiotics, particularly the flower oils against specific bacteria and also demonstrate notable antibacterial activity in various vegetable models.
  • * The essential oils show potential antitumor effects through cell viability tests, displaying both antioxidative properties and a reduction in cell migration, indicating possible application as chemotherapeutic agents in treating certain cancer cell lines.
View Article and Find Full Text PDF

Spanish marjoram () and cardamom () are traditional aromatic plants with which several pharmacological properties have been associated. In this study, the volatile composition, antioxidative and antimigratory effects on human breast cancer (MDA-MB-468 cell line), antimicrobial activity, and antibiofilm effect were evaluated. Results obtained via treatment of human breast cancer cells generally indicated an inhibitory effect of both essential oils (EOs) on cell viability (after long-term treatment) and antioxidative potential, as well as the reduction of nitric oxide levels.

View Article and Find Full Text PDF
Article Synopsis
  • * Essential oils from both locations were tested for their antitumor effects on human cancer cell lines, showing a dose-dependent reduction in cell viability, particularly in breast cancer cells.
  • * Both EOs demonstrated pro-apoptotic activity and enhanced caspase-3 levels, along with antioxidative properties, despite some recovery in cell viability after long-term exposure.
View Article and Find Full Text PDF

The present study aimed to evaluate deer meat microbiological quality when treated with essential oil (EO) from (dissolved in rapeseed oil at concentrations 0.5 and 1%), in combination with vacuum packaging during 20 days of storage of meat at 4 °C. Total viable counts (TVC), coliforms bacteria (CB), lactic acid bacteria (LAB) and spp.

View Article and Find Full Text PDF

This report summarizes the chemical composition analysis of L. flower, leaf, and stem methanol extracts (FME, LME, SME, respectively) as well as their hepatoprotective and antigenotoxic features and . Herein, Wistar rat liver intoxication with CCl resulted in the generation of trichloromethyl and trichloromethylperoxy radicals, causing lipid peroxidation within the hepatocyte membranes (viz.

View Article and Find Full Text PDF
Article Synopsis
  • The study investigates the antimetastatic effects of royal jelly and its component 10-hydroxy-2-decenoic acid (10H2DA) on colorectal cancer cells, focusing on how it can limit cell migration and invasion.
  • Analysis using HPLC found that the concentration of 10H2DA in Serbian royal jelly was 0.92%, and 10H2DA treatment did not show cytotoxic effects but significantly inhibited cancer cell motility.
  • Results indicated that 10H2DA enhanced E-cadherin levels, promoting cell adhesion and potentially preventing cancer metastasis by reinforcing intercellular connections in the HCT-116 and SW-480 cell lines.
View Article and Find Full Text PDF
Article Synopsis
  • * Researchers synthesized five series of phenolic-acylhydrazones and tested their antioxidant effects and impact on the viability of HCT-116 (cancer) and MRC-5 (normal) cells.
  • * Out of 40 compounds tested, 20 demonstrated antioxidant activity, and some showed higher selectivity in targeting cancer cells, highlighting how the structure and functional groups of these compounds influence their effectiveness.
View Article and Find Full Text PDF

In the present work we modified the procedure for isolation of naphthoquinones α-methylbutyrylshikon ( ), acetylshikonin ( ) and -hydroxyisovalerylshikonin ( ) from Clem. We also investigated possible mechanisms of , and as antitumor agents. Accordingly, we estimated concentrations of superoxide anion radical (O ), nitrite (NO ) and glutathione in HCT-116 and MDA-MB-231 cell lines.

View Article and Find Full Text PDF

Acetylshikonin (AcSh), as a red colored pigment found in roots of the plants from family , showed excellent cytotoxic activity. Due to its hydrophobic nature, and thus poor bioavailability, the aim of this study was to prepare acetylshikonin/β-cyclodextrin (AcSh/β-CD) inclusion complex by using coprecipitation method, characterize obtained system by using UV/VIS, IR and H NMR spectroscopy, and determine cytotoxic activity. Phase solubility test indicated formation of A-type binary system (substrate/ligand ratio was 1:1 M/M), with stability constant Ks of 306.

View Article and Find Full Text PDF

Isolated and structurally confirmed, eleven flavonoids from propolis were examined for their cytotoxicity toward human colon cancer and human breast cancer cells. Their effect on induction of apoptosis and their antioxidative activities were also evaluated. Six flavonoids induced cytotoxic effects in both cell lines.

View Article and Find Full Text PDF

The experimental and theoretical investigations of structure of the 3-(1-(phenylamino)ethylidene)-chroman-2,4-dione were performed. X-ray structure analysis and spectroscopic methods (FTIR and FT-Raman, H and C NMR), along with the density functional theory calculations (B3LYP functional with empirical dispersion corrections D3BJ in combination with the 6-311 + G(d,p) basis set), were used in order to characterize the molecular structure and spectroscopic behavior of the investigated coumarin derivative. Molecular docking analysis was carried out to identify the potency of inhibition of the title molecule against human's Ubiquinol-Cytochrome C Reductase Binding Protein (UQCRB) and Methylenetetrahydrofolate reductase (MTHFR).

View Article and Find Full Text PDF

In the present study, five root extracts of Onosma visianii Clem were investigated for their in vitro cytotoxic activity. On the basis of HPLC-PDA analysis, these extracts have proved to be a rich source of naphthoquinones as natural colourants for food and cosmetic industry. All investigated root extracts contain acetylshikonin, isobutyrylshikonin and α-methylbutyrylshikonin as major compounds.

View Article and Find Full Text PDF

In this study, the antibacterial and cytotoxic activities of isolated compounds from the roots of were investigated. By using different chromatographic techniques and appropriate spectroscopic methods, the seven naphthoquinones were described: deoxyshikonin ( ), isobutyrylshikonin ( ), α-methylbutyrylshikonin ( ), acetylshikonin ( ), β-hydroxyisovalerylshikonin ( ), 5,8--dimethyl isobutyrylshikonin ( ) and 5,8--dimethyl deoxyshikonin ( ). Among the tested compounds, and exhibited the highest antibacterial activities toward all tested bacterial species (MIC and MIC for gram positive bacteria: 6.

View Article and Find Full Text PDF