Protocol for a non-surgical model of perineural invasion for assessing neural drivers of cancer aggressiveness in mice.

Perineural invasion (PNI) is a significant risk factor for cancer recurrence and metastasis; however, its mechanisms relating to cancer aggressiveness remain poorly understood. Here, we present a protocol for a non-surgical model of PNI in mice using a neurotropic melanoma cell line that migrates from the skin to the sciatic nerve. We describe the steps for cell culture and injection, tumor burden measurements, mouse euthanasia, and tissue dissection.

Negative Predictive Value of Biopsy Margins in Keratinocyte Carcinoma: A Literature Review.

Background: Pathologists sometimes include commentary on margin involvement in shave biopsy reports of keratinocyte carcinoma (KC). This practice can lead to confusion regarding the need for further treatment. There is limited literature evaluating the reliability of reported histologic margin status in shave biopsies of KC.

Negative predictive value of biopsy margins of dysplastic nevi: A single-institution retrospective review.

Background: Biopsies of dysplastic nevi processed by bread-loafing allow for limited margin assessment; however, reported biopsy margins often influence management.

Objective: To evaluate the negative predictive value of biopsy margins of dysplastic nevi.

Methods: A retrospective search of a single academic institution's pathology database was conducted to identify all biopsy specimens of dysplastic nevi between January 1, 2015, and December 31, 2017.