Quality of Care of Adult Patients With Allergic Diseases in Urban, Rural, and Remote Primary Care Sites in the Philippines.

Introduction: This study determined the clinic prevalence and compared the quality of care of allergic diseases in urban, rural, and remote primary care sites.

Methods: This was a retrospective review of electronic health records of all adult patients who consulted in the 3 sites from May 2019 to April 2022. Data of adult patients with allergic diseases was extracted from the 3 EHR systems operating across the participating sites using standardized Structured Query Language queries across the 3 systems.

Absolute quantification of multisite phosphorylation by selective reaction monitoring mass spectrometry: determination of inhibitory phosphorylation status of cyclin-dependent kinases.

Multisite phosphorylation is an important mechanism for achieving intricate regulation of protein function. Here we extended the absolute quantification of abundance (AQUA) methodology and validated its applicability to quantitatively study multisite phosphorylation. As a test case, we chose the conserved inhibitory site of the cyclin-dependent kinases (CDKs), Cdk1, Cdk2, and Cdk3, which are important regulators of cell cycle transitions and apoptosis.