Inhibition of miR-33 Alleviates Inflammation Response in Ctenopharyngodon idella Kidney Cells Induced by Chloroquine/Lipopolysaccharide.

A previous study in our laboratory revealed that microRNA-33 (miR-33) regulated autophagy initiation and inflammatory response by targeting Atg5; furthermore, in this study, chloroquine (CQ), lipopolysaccharide (LPS) and the miR-33 inhibitor were transfected into Ctenopharyngodon idella kidney (CIK) cells to explore whether miR-33 regulated late-stage autophagy and inflammatory response induced by LPS. The results showed that CQ inhibited the fusion of autophagosome and lysosome and significantly increased the secretion of pro-inflammatory cytokines (p < 0.05).

MiR-33-Mediated Regulation of Autophagy and Inflammation in CIK Cells Through Atg5.

MicroRNA-33 (miR-33) plays a critical role in the regulation of autophagy and inflammatory responses. In this study, C. idella kidney (CIK) cells were transfected with a miR-33 mimic or inhibitor and Atg5 was overexpressed or silenced to elucidate the regulatory mechanism of miR-33.

Fatty hepatocytes-derived exosomal miR-122 reduces immune function and antioxidant defence in Ctenopharyngodon idella kidney (CIK) cells.

Exosomes are important for intercellular "cross talk", but the role of exosomes in communication between hepatocytes and C. idella kidney (CIK) cells remains unknown. In this study, we detected the changes in factors related to immune and oxidative stress to investigate the molecular mechanism by which fatty hepatocyte-derived exosomes (OA-Exos) reduced immunity and induced oxidative stress in CIK cells.

Anti-miR33 therapy improved hepatopancreatic lipid and immune metabolism disorders in grass carp, Ctenopharyngodon idella.

Lipid metabolism disorders are found ubiquitously in farmed fish and occur as a result of excessive fat accumulation. Previous studies have found that miR-33 is involved in lipid metabolism; however, its role in fish lipid metabolism is unclear. We sought to clarify this relationship in grass carp in vivo and in vitro.