Microwave-Ultrasound-Assisted Extraction Coupled with Natural Deep Eutectic Solvent Enables High-Yield, Low-Solvent Recovery of Curcumin from L.

: Solvent-intensive methods are traditionally required to extract curcumin, a potent bioactive compound from , raising environmental and safety concerns. : This study introduces an efficient and scalable extraction approach using microwave-ultrasound-assisted extraction (MUAE) combined with a natural deep eutectic solvent (NADES) composed of choline chloride and lactic acid. Process parameters, including solvent water content (20-30% /) and solid loading (4-8% /), were optimized using response surface methodology (RSM) to enhance curcumin yield.

Enhanced succinic acid production from lignocellulosic biomass using Actinobacillus succinogenes via semi-simultaneous saccharification and fermentation.

The utilization of semi-simultaneous saccharification and fermentation (SSSF) as the novel configuration has resulted in enhanced succinic acid (SA) production from lignocellulose biomass by Actinobacillus succinogenes. The effect of inoculum concentration, biomass type, substrate concentration, and fermentation configuration on SA production was examined in this study. The pre-hydrolysis process was applied to the pre-treated biomass for 6 h to facilitate the simultaneous saccharification and fermentation (SSF) process, which was then carried out for 48 h to achieve the SSSF configuration.

Utilizing rice straw and sugarcane bagasse as low-cost feedstocks towards sustainable production of succinic acid.

Succinic acid (SA) has been produced from rice straw (RS) and sugarcane bagasse (SB) as low-cost feedstocks in this study through sequential peracetic acid (PA) and alkaline peroxide (AP) pretreatment assisted by ultrasound and pre-hydrolysis followed by simultaneous saccharification and fermentation (PSSF). The effect of yeast extract (YE) concentration, inoculum concentration, and biomass type on SA production was investigated. The results showed that SA production from RS and SB was significantly affected by the YE concentration.

Immobilization of Cholesterol Oxidase from Streptomyces Sp. on Magnetite Silicon Dioxide by Crosslinking Method for Cholesterol Oxidation.

Enzymatic biosensor has been paid much attention to the research fields due to its advantage in medical application. As one of the application, we determined the optimum value of cholesterol oxidase against cholesterol. In this work, we studied the behavior of cholesterol oxidation by enzymatic reaction to get the optimum condition for cholesterol oxidation.