A Rapid Heat-Enhanced Golgi-Cox Staining Method for Detailed Neuroanatomical Analysis Coupled With Immunostaining.

The Golgi-Cox staining technique is renowned for its ability to delineate neuronal architecture with remarkable precision. However, the traditional protocol's lengthy processing timeline and limited compatibility with immunostaining and transgenic labeling have hindered its widespread adoption in modern neuroscience research. In the current study, we found that adjusting the incubation temperature to 55°C significantly reduced the staining duration to a mere 24 h for 100 µm-thick sections of mouse brain tissue.