Publications by authors named "Matthieu Chourrout"
Article Synopsis
- Myocardial infarction (MI) causes heart muscle damage due to blocked blood flow, leading to the production of reactive oxygen species and a redox imbalance, with myoglobin playing a key role in this process.
- The study introduces a new imaging method that uses advanced techniques to examine the oxidation-reduction states of myoglobin in heart tissue after MI, revealing how myoglobin's fluorescence can indicate the state of the myocardium.
- Findings show that the spectral properties of myoglobin in infarcted heart tissue correlate with infarct size, suggesting myoglobin's redox state could be a valuable biomarker for assessing MI severity in its early stages.
Amyloid-β (Aβ) plaques from Alzheimer's Disease (AD) can be visualized ex vivo in label-free brain samples using synchrotron X-ray phase-contrast tomography (XPCT). However, for XPCT to be useful as a screening method for amyloid pathology, it is essential to understand which factors drive the detection of Aβ plaques. The current study was designed to test the hypothesis that Aβ-related contrast in XPCT could be caused by Aβ fibrils and/or by metals trapped in the plaques.
View Article and Find Full Text PDFTo improve our understanding of the brain microstructure, high-resolution 3D imaging is used to complement classical 2D histological assessment techniques. X-ray computed tomography allows high-resolution 3D imaging, but requires methods for enhancing contrast of soft tissues. Applying contrast-enhancing staining agents (CESAs) ameliorates the X-ray attenuating properties of soft tissue constituents and is referred to as contrast-enhanced computed tomography (CECT).
View Article and Find Full Text PDFWhite-matter injury leads to severe functional loss in many neurological diseases. Myelin staining on histological samples is the most common technique to investigate white-matter fibers. However, tissue processing and sectioning may affect the reliability of 3D volumetric assessments.
View Article and Find Full Text PDFWhile numerous transgenic mouse strains have been produced to model the formation of amyloid-β (Aβ) plaques in the brain, efficient methods for whole-brain 3D analysis of Aβ deposits have to be validated and standardized. Moreover, routine immunohistochemistry performed on brain slices precludes any shape analysis of Aβ plaques, or require complex procedures for serial acquisition and reconstruction. The present study shows how in-line (propagation-based) X-ray phase-contrast tomography (XPCT) combined with ethanol-induced brain sample dehydration enables hippocampus-wide detection and morphometric analysis of Aβ plaques.
View Article and Find Full Text PDFArticle Synopsis
- - An observational study was conducted using a rat model of ischemic stroke to design a preclinical study for an intracerebral cell-based therapy, focusing on neurofunctional and imaging outcomes shortly after the stroke.
- - Key findings indicated that side bias and axial diffusivity (AD) were the most important measures, with variations depending on the lesion type: hypothalamic, striatal, or corticostriatal.
- - It was confirmed that intracerebral administration did not negatively affect the outcomes, and sample size calculations suggest that a future therapeutic trial would require a multicenter approach with 18-147 rats per group based on the chosen endpoint.