Publications by authors named "Manuel Pires-Santos"
The encapsulation of single cells has emerged as a promising field in recent years, owing to its potential applications in cell-based therapeutics, bioprinting, in vitro cell culture, high-throughput screening, and diagnostics. Single-cell units offer several advantages, including compatibility with standard imaging techniques, superior diffusion rates, and lower material-to-cell volume ratios. They also serve as effective carriers for targeted drug delivery, allowing precise administration of therapeutics in cell-mediated quantities.
View Article and Find Full Text PDFMore recently, single-cell encapsulation emerged as a promising field in biomedicine due to its potential applications, in cell analysis and therapy. Traditional techniques involve embedding cells in crosslinked polymers to create continuous microgels, suitable mainly for adherent cells, or encapsulating them in droplets for only short-term analysis, due to their instability. In this study, we developed a method for encapsulating single cells in liquid-core microcapsules to address these limitations.
View Article and Find Full Text PDFBackground: Surface topography has been shown to influence cell behavior and direct stromal cell differentiation into distinct lineages. Whereas this phenomenon has been verified in two-dimensional cultures, there is an urgent need for a thorough investigation of topography's role within a three-dimensional (3D) environment, as it better replicates the natural cellular environment.
Methods: A co-culture of Wharton's jelly-derived mesenchymal stem/stromal cells (WJ-MSCs) and human umbilical vein endothelial cells (HUVECs) was encapsulated in a 3D system consisting of a permselective liquefied environment containing freely dispersed spherical microparticles (spheres) or nanogrooved microdiscs (microdiscs).