Publications by authors named "Manoj B Pohare"
Flap endonuclease (FEN) enzymes are a group of metallonucleases that have essential roles in DNA repair and the maintenance of genomic resilience. They catalyse hydrolytic cleavage of a phosphodiester bond to remove 5'-flaps present on double-stranded DNA molecules formed during DNA replication. FEN locates a target scissile bond through the structural recognition of a pronounced bend in the double-stranded DNA substrate along with the presence of both a 5'-flap and a 1-nucleotide 3'-flap.
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- The study focuses on the SARS-CoV-2 nucleocapsid protein (Ncap), which is important for diagnostics and vaccine development since the Covid-19 pandemic started in 2019.
- Researchers successfully expressed and purified SARS-CoV-2 Ncap proteins in E. coli, specifically using a synthetic gene with a 6His affinity tag to enhance purification.
- ELISA assays were conducted to compare the immune response of human sera to different Ncap proteins, showing no major differences between tagged and untagged versions, except a slight potential decrease in sensitivity to some Ncap variants.