Kueselia aquadivae gen. nov., sp. nov., the first member of the family Isosphaeraceae isolated from subsurface percolates.

Subsurface habitats, found under various geological conditions, exhibit diverse microbial communities. The vadose zone, a previously unexplored subsurface compartment, connects the surface to phreatic groundwater. Drilling into the subsurface allows access to these habitats for microbial diversity study.