Steam sterilisation of disposable surgical gowns does not compromise resistance to wet microbial penetration.

Background: Reuse of disposable medical products is common practice in veterinary medicine; however, studies on the safety of such reuse are often lacking. This study aimed to determine whether steam sterilisation of disposable surgical gowns compromises their resistance to wet microbial penetration.

Methods: Twenty surgical gowns were removed from their sterile packaging, manipulated, wetted, dried and subsequently repacked and steam sterilised.

Computed tomographic evaluation of the proximity of needles placed for perineural anesthesia of the palmar digital nerves to synovial structures in the foot: an study.

Background: Potential synovial penetration following palmar digital nerve blocks has not been investigated.

Objectives: To evaluate the proximity of needles placed for palmar digital nerve blocks to nearby synovial structures using computed tomography (CT).

Study Design: Descriptive observational study.

Longitudinal pilot study examining the effect of punch biopsy on equine sarcoid growth dynamics.

Background: Nonexcisional tissue biopsies facilitate pre-operative confirmation of equine sarcoid yet fear of lesion deterioration currently limits its use in the diagnostic workup.

Objectives: To evaluate the effect of a single punch biopsy on tumour growth dynamics [thickness, area, circumference, viral load (VL) and Visual Analog Scale (VAS)].

Animals: Six client-owned horses with 11 sarcoids of various classification.

Dobutamine use in horses during romifidine and isoflurane anaesthesia.

This retrospective study aimed to assess the incidence of hypotension and the subsequent administration of dobutamine in horses anesthetized with isoflurane and romifidine during elective surgery. Time from induction of anaesthesia to administration of dobutamine was registered, as well as the time and dose needed to restore mean arterial pressure (MAP) ≥ 70 mmHg. Additionally, the influence of patient and anaesthesia related parameters on the need for dobutamine supplementation was evaluated.

CRISPR/Cas9-Mediated Targeting of BPV-1-Transformed Primary Equine Sarcoid Fibroblasts.

Equine sarcoids (EqS) are fibroblast-derived skin tumors associated with bovine papillomavirus 1 and 2 (BPV-1 and -2). Based on Southern blotting, the BPV-1 genome was not found to be integrated in the host cell genome, suggesting that EqS pathogenesis does not result from insertional mutagenesis. Hence, CRISPR/Cas9 implies an interesting tool for selectively targeting BPV-1 episomes or genetically anchored suspected host factors.

The effect of medical grade honey on tensile strength, strain, and Young's modulus of synthetic absorbable suture material used in equine surgery.

Background: Medical grade honey (MGH) has been introduced as a protective measure against surgical site infections in equine surgery, but the effect on suture material has not been investigated.

Objective: To evaluate the effect of MGH on the tensile properties of three synthetic absorbable suture materials.

Study Design: In vitro experiments.

Immunogenicity analysis of BPV-1 positive equine sarcoid-derived cultured fibroblasts.

Sarcoids are the most common equine skin tumours Although they do not metastasize, they can be locally aggressive and cause significant clinical symptoms in affected horses. Despite being common, very little is known about the host immune response and the biological mechanisms underlying persistence and recurrence of equine sarcoids. The latter reflects the need for further research in this field.

Different local, innate and adaptive immune responses are induced by two commercial bacterins and an adjuvant alone.

Introduction: Enzootic pneumonia still causes major economic losses to the intensive pig production. Vaccination against its primary pathogen, , is carried out worldwide to control the disease and minimize clinical signs and performance losses. Nonetheless, the effects of both infection with, and vaccination against on the innate and adaptive immune responses remain largely unknown.

Cross-sectional comparison of superficial swab and fine-needle aspiration: Improving the diagnostic workup of horses with sarcoids.

Although bovine papillomavirus (BPV)-induced equine sarcoids are often identified solely by clinical examination, confirmation of the clinical diagnosis is essential for correct treatment selection. Only few approaches are presently available for this purpose. PCR-based screening for BPV in superficial swabs is widely-used to support clinical suspicion.

New approach for genomic characterisation of equine sarcoid-derived BPV-1/-2 using nanopore-based sequencing.

Background: Bovine papillomavirus (BPV) types 1 and 2 play a central role in the etiology of the most common neoplasm in horses, the equine sarcoid. The unknown mechanism behind the unique variety in clinical presentation on the one hand and the host dependent clinical outcome of BPV-1 infection on the other hand indicate the involvement of additional factors. Earlier studies have reported the potential functional significance of intratypic sequence variants, along with the existence of sarcoid-sourced BPV variants.

Limited added value of negative pressure wound therapy compared with calcium alginate dressings for second intention healing in a noncontaminated and contaminated equine distal limb wound model.

Background: Distal limb wounds in horses often show aberrant healing due to a slow inflammatory response. In human medicine, negative pressure wound therapy (NPWT) is used for the treatment of chronic wounds with a similar inflammatory response.

Objectives: To compare the effect of NPWT to calcium alginate dressings on the healing of (non) contaminated equine distal limb wounds.

Clinical applicability of detomidine and methadone constant rate infusions for surgery in standing horses.

Objective: To determine the required rate of a detomidine infusion (loading dose 5 μg kg; initial rate 12.5 μg kg hour) added to a constant infusion of methadone (0.2 mg kg; 0.

Skin disease in donkeys (Equus asinus): a retrospective study from four veterinary schools.

Background: Donkeys are important throughout the world as work animals and occasionally as pets or a meat source. Most descriptions of skin disease in donkeys are reported in small case series, textbooks or review articles.

Hypothesis/objectives: To document skin diseases and their prevalence in donkeys and to investigate predilections for the most common conditions.

Surgical treatment of epiploic foramen entrapment in 142 horses (2008-2016).

Objective: To report the clinical features, outcomes, and prognostic factors associated with the surgical treatment of epiploic foramen entrapment (EFE).

Study Design: Retrospective study at a single referral hospital.

Animals: Horses (n = 142) undergoing surgery (n = 145) for EFE.

Anatomy of the vestibulum esophagi and surgical implications during prosthetic laryngoplasty in horses.

Objective: To describe the anatomy of the entry to the equine esophagus (vestibulum esophagi) and to assess the risk of penetrating its adventitia and/or lumen during laryngoplasty.

Study Design: Ex vivo cadaveric study.

Sample Population: Five isolated equine larynges and 39 equine head and neck specimens.

Incidence and risk factors of surgical site infection and septic arthritis after elective arthroscopy in horses.

Objective: To determine the incidence of infection and associated risk factors, after elective arthroscopy.

Study Design: Retrospective case study.

Animals: Horses (n=1079) undergoing elective arthroscopy.

Topical use of 5% acyclovir cream for the treatment of occult and verrucous equine sarcoids: a double-blinded placebo-controlled study.

Background: Previous studies mention the use of topical acyclovir for the treatment of equine sarcoids. Success rates vary and since the bovine papillomavirus (BPV) lacks the presence of a kinase necessary to activate acyclovir, there is no proof of its activity against equine sarcoids.

Results: Twenty-four equine sarcoids were topically treated with acyclovir cream and 25 with a placebo.

Comparison of antibacterial effects among three foams used with negative pressure wound therapy in an ex vivo equine perfused wound model.

OBJECTIVE To compare antibacterial effects among 3 types of foam used with negative-pressure wound therapy (NPWT) in an ex vivo equine perfused wound model. SAMPLES Abdominal musculocutaneous flaps from 6 equine cadavers. PROCEDURES Each musculocutaneous flap was continuously perfused with saline (0.

Treatment of sarcoids in equids: 230 cases (2008-2013).

OBJECTIVE To evaluate outcomes following treatment of sarcoids in equids and to identify risk factors for treatment failure in these patients. DESIGN Retrospective case series. ANIMALS 230 equids with 614 sarcoids.