Capturing G protein-coupled receptors into native lipid-bilayer nanodiscs using new diisobutylene/maleic acid (DIBMA) copolymers.

Many membrane proteins, including G protein-coupled receptors (GPCRs), are susceptible to denaturation when extracted from their native membrane by detergents. Therefore, alternative methods have been developed, including amphiphilic copolymers that enable the direct extraction of functional membrane proteins along with their surrounding lipids. Among these amphiphilic copolymers, styrene/maleic acid (SMA) and diisobutylene/maleic acid (DIBMA) polymers have been extensively studied.

Heterologous expression and immunological reactivity of the recombinant lipoprotein GUDIV-517 from Ureaplasma diversum.

Ureaplasma diversum infects cattle and plays a significant role in economic losses in the livestock sector, as it is associated with the development of reproductive and respiratory disorders in these animals. Studies have suggested that membrane-associated lipoproteins (LAMPs) are closely linked to the pathogenicity of these bacteria. Thus, this study aimed to express the lipoprotein GUDIV-517 from U.

Biallelic variants in cause the most prevalent known recessive neurodevelopmental disorder.

We recently showed that mutations in and , two genes that are transcribed into small nuclear RNA (snRNA) components of the major spliceosome, are prevalent causes of dominant neurodevelopmental disorders (NDDs). By genetic association comparing 12,776 NDD cases with 56,064 controls, we now demonstrate the existence of a recessive form of syndrome that, in England, is even more common than the dominant form. We inferred log Bayes factors for dominant and recessive models of association of 14.

Controlling Magnetic Anisotropy of Endohedral Lanthanide Ions by Carbene Addition: Paramagnetic NMR, Lanthanide Luminescence, and Single-Molecule Magnetism in Adamantylidene Adducts of MScN@C (M = Nd, Dy).

Metallofullerenes with endohedral lanthanides have emerged as a versatile class of single-molecule magnets owing to strong single-ion magnetic anisotropy, which can be realized in the interior of the fullerene cage. Since exohedral chemical modification of fullerenes is often used to adjust their properties and processability for prospective practical applications, it is necessary to understand how it can affect their magnetic properties. In this work, we studied how a popular [2 + 1] cycloaddition reaction, photochemical addition of adamantylidene (Ad), affects single-ion magnetic anisotropy and single-molecule magnetism of MScN@C (M = Nd, Dy).

International Pain Outcomes (IPO) - Brazilian Portuguese version: cross-cultural adaptation and psychometric validation.

Objectives: to adapt the International Pain Outcomes questionnaire into Brazilian Portuguese and evaluate its clinimetric properties.

Methods: we conducted a methodological, quantitative, validation, and cross-cultural adaptation study of the instrument. The process included translation, synthesis, expert committee review, back-translation, consensus between the English versions and comparison with the original version, pretest phase (n=30), and metric property validation (convergent validity, n=360; interobserver reliability analysis, n=54; internal consistency, n=360).