Filopodia numbers impact chemotactic migration speed.

Migrating cells sense and respond to external chemical and physical cues, enabling them to efficiently reach their destinations. Filopodia are slender actin-filled membrane protrusions implicated in interacting with the extracellular environment in many contexts, such as neuronal growth cone guidance and the capture of prey by immune cells and unicellular organisms. The role of filopodia in chemotactic guidance in fast-moving amoeboid cells has not been well-studied.

Woronin body hitchhiking on early endosomes is dispensable for septal localization in .

The proper functioning of organelles depends on their intracellular localization, mediated by motor protein-dependent transport on cytoskeletal tracks. Rather than directly associating with a motor protein, peroxisomes move by hitchhiking on motile early endosomes in the filamentous fungus . However, the physiological role of peroxisome hitchhiking is unclear.

Woronin bodies move dynamically and bidirectionally by hitchhiking on early endosomes in .

The proper functioning of organelles depends on their intracellular localization, mediated by motor protein-dependent transport on cytoskeletal tracks. Rather than directly associating with a motor protein, peroxisomes move by hitchhiking on motile early endosomes in the filamentous fungus . However, the cellular function of peroxisome hitchhiking is unclear.

PxdA interacts with the DipA phosphatase to regulate peroxisome hitchhiking on early endosomes.

In canonical microtubule-based transport, adaptor proteins link cargoes to dynein and kinesin motors. Recently, an alternative mode of transport known as "hitchhiking" was discovered, where cargoes achieve motility by hitching a ride on already-motile cargoes, rather than attaching to a motor protein. Hitchhiking has been best studied in two filamentous fungi, and In ribonucleoprotein complexes, peroxisomes, lipid droplets (LDs), and endoplasmic reticulum hitchhike on early endosomes (EEs).

Optimized filopodia formation requires myosin tail domain cooperation.

Filopodia are actin-filled protrusions employed by cells to interact with their environment. Filopodia formation in Amoebozoa and Metazoa requires the phylogenetically diverse MyTH4-FERM (MF) myosins DdMyo7 and Myo10, respectively. While Myo10 is known to form antiparallel dimers, DdMyo7 lacks a coiled-coil domain in its proximal tail region, raising the question of how such divergent motors perform the same function.