Eryptosis in Liver Diseases: Contribution to Anemia and Hypercoagulation.

Eryptosis is a type of regulated cell death of mature erythrocytes characterized by excessive Ca accumulation followed by phosphatidylserine externalization. Eryptosis facilitates erythrophagocytosis resulting in eradication of damaged erythrocytes, which maintains the population of healthy erythrocytes in blood. Over recent years, a wide array of diseases has been reported to be linked to accelerated eryptosis, which leads to anemia.

MnO Nanocrystal-Induced Eryptosis Features Ca Overload, ROS and RNS Accumulation, Calpain Activation, Recruitment of Caspases, and Changes in the Lipid Order of Cell Membranes.

Accumulating evidence suggests that manganese oxide nanoparticles (NPs) show multiple enzyme-mimicking antioxidant activities, which supports their potential in redox-targeting therapeutic strategies for diseases with impaired redox signaling. However, the systemic administration of any NP requires thorough hemocompatibility testing. In this study, we assessed the hemocompatibility of synthesized MnO NPs, identifying their ability to induce spontaneous hemolysis and eryptosis or impair osmotic fragility.

Urinary Diversion as Last Option After Failed Artificial Urinary Sphincter and Iatrogenic Devastated Bladder Outlet.

Objectives: To describe functional outcomes and identify risk factors for artificial urinary sphincter (AUS) failure and need for urinary diversion (UD) in patients with iatrogenic devastated bladder outlet (DBO). Our hypothesis was that pelvic radiation or ablative therapy (RT/AT) increases the risk of complications compared to surgery alone.

Methods: Multicenter, retrospective study of patients with complications from previous treatments, subsequent AUS failure and unsalvageable DBO, ultimately requiring UD between 2008 and 2020.

Nanostructured zinc carbonate hydroxide microflakes: assessing the toxicity against erythrocytes and L929 cells.

Nanostructured materials have been suggested to be used as a source of dietary zinc for livestock animals. In this study, we assessed the cytotoxicity of newly synthesized nanostructured zinc carbonate hydroxide (ZnCH) Zn(CO)(OH)microflakes. Cytotoxicity of the microflakes was assessed against murine L929 cell line and rat mature erythrocytes.

GdVO:Eu and LaVO:Eu Nanoparticles Exacerbate Oxidative Stress in L929 Cells: Potential Implications for Cancer Therapy.

The therapeutic potential of redox-active nanoscale materials as antioxidant- or reactive oxygen species (ROS)-inducing agents was intensely studied. Herein, we demonstrate that the synthesized and characterized GdVO:Eu and LaVO:Eu nanoparticles, which have been already shown to have redox-active, anti-inflammatory, antibacterial, and wound healing properties, both in vitro and in vivo, worsen oxidative stress of L929 cells triggered by hydrogen peroxide or -butyl hydroperoxide (tBuOOH) at the concentrations that are safe for intact L929 cells. This effect was observed upon internalization of the investigated nanosized materials and is associated with the cleavage of caspase-3 and caspase-9 without recruitment of caspase-8.

Oxidative stress-modifying effects of TiOnanoparticles with varying content of Ti(Ti) ions.

Nanoparticles (NPs) with reactive oxygen species (ROS)-regulating ability have recently attracted great attention as promising agents for nanomedicine. In the present study, we have analyzed the effects of TiOdefect structure related to the presence of stoichiometric (Ti) and non-stoichiometric (Tiand Ti) titanium ions in the crystal lattice and TiONPs aggregation ability on HO- and tert-butyl hydroperoxide (tBOOH)-induced ROS production in L929 cells. Synthesized TiO-A, TiO-B, and TiO-C NPs with varying Ti(Ti) content were characterized by x-ray powder diffraction, transmission electron microscopy, small-angle x-ray scattering, x-ray photoelectron spectroscopy, and optical spectroscopy methods.

Marine Polysaccharides Carrageenans Enhance Eryptosis and Alter Lipid Order of Cell Membranes in Erythrocytes.

Unlabelled: Aim In the current study, hemocompatibility of three major commercially available types of carrageenans (ι, κ and λ) was investigated focusing on eryptosis.

Materials And Methods: Carrageenans of ι-, κ- and λ-types were incubated with washed erythrocytes (hematocrit 0.4%) at 0-1-5-10 g/L for either 24 h or 48 h.

Hemocompatibility of dextran-graft-polyacrylamide/zinc oxide nanosystems: hemolysis or eryptosis?

. In this study, blood compatibility of ZnO nanoparticles-polymer nanocomplex (D-PAA/ZnONPs(SO42-)) synthesizedinto dextran-graft-polyacrylamide (D-PAA) using zinc sulphate as a precursor was tested using hemolysis, osmotic fragility and eryptosis assays..

Rare-earth orthovanadate nanoparticles trigger Ca-dependent eryptosis.

. Rare-earth orthovanadate nanoparticles (ReVO:Eu, Re = Gd, Y or La) are promising agents for photodynamic therapy of cancer due to their modifiable redox properties. However, their toxicity limits their application.