Publications by authors named "Lessly P Sepulveda-Rincon"
Background: Male germline stem cells are relevant for stem cell researchers but also for andrologists as they are crucial for testis function and initiation/maintenance of spermatogenesis. They are also considered a target for fertility preservation in the male; e.g.
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- Primordial germ cells (PGCs) are the earliest forms of potential gametes, typically developing into oocytes or sperm but can become pluripotent in certain conditions.
- Research shows that when PGCs are injected into 8-cell embryos, they can survive and contribute to the inner cell mass initially, particularly showing enhanced survival when apoptosis is inhibited.
- Despite their initial contributions, PGCs have limited ability to integrate into the post-implantation embryo, while PGC-like cells show greater potential to contribute to mid-gestation chimeras, indicating a form of latent pluripotency in PGCs.
The first lineage specification during mammalian embryo development can be visually distinguished at the blastocyst stage. Two cell lineages are observed on the embryonic-abembryonic axis of the blastocyst: the inner cell mass and the trophectoderm. The timing and mechanisms driving this process are still not fully understood.
View Article and Find Full Text PDFDespite ongoing research in a number of species, the efficiency of embryo production by nuclear transfer remains low. Incomplete epigenetic reprogramming of the nucleus introduced in the recipient oocyte is one factor proposed to limit the success of this technique. Nonetheless, knowledge of reprogramming factors has increased-thanks to comparative studies on reprogramming of the paternal genome brought by sperm on fertilization-and will be reviewed here.
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