Immune landscape and TAM density in endometrial cancer: implications for immune checkpoint inhibitors efficacy.

Background: Immune checkpoint inhibitors (ICIs) have shown efficacy in endometrial cancer (EC); however, their efficacy varies according to mismatch repair (MMR) status. Notably, even among patients with MMR-deficient (MMRd) or microsatellite instability-high (MSI-H) tumors, approximately one-third exhibit primary resistance to ICI monotherapy.

Objectives: We aimed to characterize dissimilarities in the tumor immune microenvironment of ICI-treated MMRd/MSI-H versus MMR-proficient (MMRp)/microsatellite stable (MSS) EC, and to identify mechanisms of resistance.

Video microscopy: an old story with a bright biological future.

Single-cell analysis is increasingly popular in the field of biology, enabling more precise analyses of heterogeneous phenomena, particularly in the fields of embryology and the study of different diseases. At the heart of this evolution is video microscopy, an ancient but revolutionary technique. From its first use on embryos, through the study of C.

The roles of AKT isoforms in decidualization and embryo implantation using a Progesterone Receptor-Cre mouse model†.

Implantation is a complex process requiring a prepared, receptive endometrium, reliant on synchronized decidualization of stromal cells. During this process, cell proliferation and apoptosis are tightly regulated by signaling factors, including the survival and proliferation of the PI3K/AKT pathway. The three AKT isoforms each play distinct physiological roles, but their specific functions in endometrial cell survival and apoptosis remain unclear.

Toto-Cell: A new software to analyze cellular events during video-microscopy.

Video-microscopy is a technology widely used to follow, in a single cell manner, cell behavior. A number of new studies are searching a way to track these behaviors by artificial intelligence; unfortunately some real-time events still have to be track manually. For that reason, we developed a software that helps the experimenter to analyze collected data.

Redundant roles of AKT1 and AKT2 in fertility, estrous cyclicity and endometrial gland development.

In Brief: The regulation of AKT in the endometrium during many cellular processes such as apoptosis and cell survival is crucial during the estrous cycle to ensure fertility. This research shows the specific function of AKT isoforms in the mouse endometrium for litter size, estrous cyclicity and endometrial gland development.

Abstract: Apoptosis and cell survival regulation are crucial processes during the estrous cycle to prepare a receptive uterus during implantation for successful recognition of pregnancy.