Publications by authors named "Lance D Reynolds"
Nonhomologous end joining (NHEJ) is required for repairing DNA double strand breaks (DSBs) generated by the RAG endonuclease during lymphocyte antigen receptor gene assembly by V(D)J recombination. The ataxia telangiectasia-mutated (ATM) and DNA-dependent protein kinase catalytic subunit (DNA-PKcs) kinases regulate functionally redundant pathways required for NHEJ. Here, we report that loss of the senataxin helicase leads to a strong defect in RAG DSB repair upon inactivation of DNA-PKcs.
View Article and Find Full Text PDFArticle Synopsis
- Non-homologous end joining (NHEJ) is a crucial process for fixing DNA double strand breaks during the V(D)J recombination in immune cells.
- The study finds that the loss of the senataxin helicase leads to difficulties in repairing these breaks, particularly when the DNA-PKcs is inactive.
- It also reveals that senataxin works closely with other helicases like RECQL5 and translocases like HLTF, suggesting multiple backup systems are in place for effective NHEJ repair involving ATM and DNA-PKcs.