Evaluating the Cytotoxic, Genotoxic, and Toxic Potential of Pyrolytic Tire Char Using Human Lymphocytes and a Bacterial Biosensor.

Waste tires (WTs) constitute a potentially significant source of pollution, and the large quantities that are disposed of require proper handling. Pyrolysis has emerged as an environmentally friendly and effective method for WT treatment. In the present study, the cyto-genotoxic and toxic effects of untreated and acid-treated pyrolytic tire char (PTC and PTC, respectively) were investigated.

Flame-retardant Tris(2-chloroethyl) phosphate: Assessing the effects on microalgae, mussel hemocytes and human peripheral blood cells.

Tris (2-chloroethyl) phosphate (TCEP) is a widely used flame retardant in numerous commercial and industrial products. Due to its widespread release and detection in various environmental matrices, TCEP has raised great concerns about its risk to aquatic biota and human health. To this end, the present study investigates the TCEP environmental and human health mediated effects on aquatic biological species/models belonging to different trophic levels, as well as on human peripheral blood lymphocytes.

Phytochemical Profile and Evaluation of the Antioxidant, Cyto-Genotoxic, and Antigenotoxic Potential of Hydromethanolic Extract.

This study comprises the phytochemical characterization, the evaluation of the total phenolic content (TPC) and antioxidant activity (AA), and the investigation of the cyto-genotoxic and antigenotoxic potential of hydromethanolic extract derived from L. leaves. HPLC-DAD-ESI-MS and HPLC-DAD were used for the characterization of the extract and determination of the major ingredients.

Isothiocyanates Potentiate Tazemetostat-Induced Apoptosis by Modulating the Expression of Apoptotic Genes, Members of Polycomb Repressive Complex 2, and Levels of Tri-Methylating Lysine 27 at Histone 3 in Human Malignant Melanoma Cells.

In this study, we utilized an in vitro model consisting of human malignant melanoma as well as non-tumorigenic immortalized keratinocyte cells with the aim of characterizing the therapeutic effectiveness of the clinical epigenetic drug Tazemetostat alone or in combination with various isothiocyanates. In doing so, we assessed markers of cell viability, apoptotic induction, and expression levels of key proteins capable of mediating the therapeutic response. Our data indicated, for the first time, that Tazemetostat caused a significant decrease in viability levels of malignant melanoma cells in a dose- and time-dependent manner via the induction of apoptosis, while non-malignant keratinocytes were more resistant.