Publications by authors named "Lakshmi Sujatha Guthula"
The highly prevalent single nucleotide polymorphism (SNP, rs671) of the aldehyde dehydrogenase (ALDH2) gene in Asian populations instigates various human pathologies and thus accentuates the urgent need for effective diagnostic tools. In this study, we present an ultrasensitive biosensing method by a combination of clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a with the fiber optic nanogold-linked sorbent assay (FONLISA) for precise SNP identification. This method leverages the sequence-specific recognition capability of the CRISPR/Cas system and the ultrahigh sensitivity via the dual signal enhancement mechanisms by integrating the trans-cleavage mechanism of Cas12a to amplify the signal from an activity reporter and the subsequent waveguide-enhanced nanoplasmonic absorption by a signaling reporter.
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- A new method utilizing promoter hypermethylation of the SOCS-1 tumor suppressor gene shows promise for early diagnosis of gastric cancer.
- Researchers developed a fiber optic nanoplasmonic biosensor that can detect methylation in DNA without the need for PCR, utilizing PCR-free DNA from gastric tumor tissue and cell lines.
- The biosensor demonstrated high sensitivity with a detection limit of 0.81 fM for methylated DNA and provided results within 15 minutes, offering a more accurate alternative to traditional PCR methods for evaluating methylation status in cancer patients.