Influence of Starch-Granule Diameter on Lasing Thresholds of the Molecular Rotor Thioflavin T in a Dual-Cavity Fabry-Perot/Whispering Gallery Mode.

A dual-cavity lasing platform is reported in which thioflavin T (ThT), a rotor-sensitive molecular probe, is employed to map molecular-crowding effects within starch granules via coupled Fabry-Perot (FP) and whispering gallery mode (WGM) resonances. In this architecture, global standing-wave feedback is furnished by a planar FP cavity, while size-tunable WGMs are supported by ThT-coated starch granules. Granules were sorted into five diameter classes (<20, 20-30, 30-40, 40-60, and >60 μm), and lasing thresholds alongside fluorescence lifetimes were determined.

Thioflavin T Lasing Probe for Mucin Detection in Simulated Tears as a Targeting Strategy for Brain Tumors.

A novel optical approach for a noninvasive detection of mucins in tear fluid is proposed, aiming at the early diagnosis of brain tumors such as glioblastoma. Utilizing Thioflavin T (ThT) as a fluorescent probe, our study demonstrates that ThT selectively binds to mucins (modeled by MUC3) in DEMI water, artificial tears, and simulated tears. Steady-state and time-resolved fluorescence spectroscopy reveal that mucin binding induces a significant enhancement in ThT fluorescence and prolonged emission lifetime, indicative of restricted intramolecular rotation.

Thioflavin T─a Reporter of Microviscosity in Protein Aggregation Process: The Study Case of α-Synuclein.

Thioflavin T (ThT) informed microviscosity changes can be used to monitor protein aggregation. Steady-state, time-resolved and lasing spectroscopy were used to detect transient states in α-synuclein - a protein associated with Parkinson's disease. The major focus was on the nucleation phase, where conventional ThT fluorescence assay lacks appropriate sensitivity to detect early stage oligomers.