Aquaporins in bovine male reproductive tract: From calves to reproductive bulls.

The role of aquaporins (AQPs) in the male reproductive system of cattle, particularly in relation to spermatogenesis, remains incompletely understood. Therefore, the aim of this study was to analyze the developmental changes in AQP8 and AQP11 expression in the bovine (Bos taurus) male reproductive tract. Tissue samples were collected from three age groups of animals: calves aged 5-6 weeks (n = 10), young bulls aged 15-25 weeks (n = 10) and reproductive bulls aged 2-6 years (n = 11).

A Step Forward in Understanding the Expression of Classical Aquaporins in the Male Reproductive Tract: Study Findings in Cattle ().

Aquaporins (AQPs), also known as water channels, appear to be particularly promising in maintaining male reproductive potential. Therefore, this study aimed to determine the presence of classical AQPs in the bovine () reproductive system and analyze changes in their expression with age using immunohistochemistry and Western blotting. Of the six classical AQPs, AQP0, AQP1, AQP4, AQP5 and AQP6 were detected, while AQP2 was absent.

Ultrastructure of the Bovine Testis in Cattle (): New View.

The purpose of this study was to analyze the ultrastructure of the testes of sexually immature calves and reproductive bulls of the Polish Holstein-Friesian Black-and-White breed. Utilizing TEM, this study identified three distinct stages of seminiferous tubule development in calves, characterized by varying shapes, distributions, and arrangements of individual cells. In immature animals, early developing spermatocytes, prespermatogonia, and pre-Sertoli cells were observed within the seminiferous tubules.

Cellular Distribution of Aquaporin 3, 7 and 9 in the Male Reproductive System: A Lesson from Bovine Study ().

The increasing incidence of male infertility in humans and animals creates the need to search for new factors that significantly affect the course of reproductive processes. Therefore, the aim of this study was to determine the temporospatial expression of aquaglyceroporins (AQP3, AQP7 and AQP9) in the bovine () reproductive system using immunohistochemistry and Western blotting. The study also included morphological analysis and identification of GATA-4.

Kidney morphology and renal expression of aquaporins 2, 3 and 4 during cerulein - Induced chronic pancreatitis in pigs.

Purpose: Chronic pancreatitis (CP) is associated with serious complications and reduced quality of life. Kidney failure is a frequent complication of acute pancreatitis (AP), however limited information is available regarding the impact of CP on this condition. In the kidney, 9 aquaporins (AQPs) are expressed to maintain body water homeostasis and concentrate urine.

Aquaporins in the male reproductive system: A chance for paternity or a road to nowhere?

Aquaporins-small, "unusual" proteins, whose discovery revolutionized the view of membrane transport of water and other small molecules, are essential for all living organisms. Aquaporins located in the male reproductive system seem to play a key role in the proper course of many processes occurring within it, thus maintaining a high reproductive potential.

Aquaporins: New markers for male (in)fertility in livestock and poultry?

Improving the methods utilized to facilitate reproduction is associated with a constant need to search for new factors that not only significantly affect reproductive processes, but also create new possibilities when assessing male reproductive potential. Aquaporins (AQPs) belong to a family of small (28-30 kDa) proteins that facilitate the transport of water and other small molecules. There have been 13 AQPs (AQP0-AQP12) discovered in mammals, and these proteins are present in a wide range of cell types.

Initial assessment of suitability of MCF-7 and HepG2 cancer cell lines for AQP3 research in cancer biology.

Cancer cell lines are widely used as in vitro models to elucidate biological processes in cancer, and as a tool to evaluate anticancer agents. In fact, the use of an appropriate cancer cell line in cancer research is crucial for investigating new, potential factors involved in carcinogenesis. One of them is aquaporin-3 (AQP3), which is a small, hydrophobic, integral membrane protein with a predominant role in water and glycerol transport.

The long-term effects of rapamycin-based immunosuppressive protocols on the expression of renal aquaporins 1, 2, 3 and 4 water channels in rats.

Background: To this day, the effect of multi-drug immunosuppressive protocols on renal expression of AQPs is unknown. This study aimed to determine the influence of rapamycin-based multi-drug immunosuppressive regimens on the expression of aquaporins (AQPs) 1, 2, 3, and 4 in the rat kidney.

Methods: For 6 months, 24 male Wistar rats were administered immunosuppressants, according to the three-drug protocols used in patients after organ transplantation.

Effects of Three-Month Feeding High Fat Diets with Different Fatty Acid Composition on Myocardial Proteome in Mice.

Westernized diet is characterized by a high content of saturated fatty acids (SFA) and a low level of omega-3 polyunsaturated fatty acids (PUFA), often accompanied by an imbalance in the omega-6/omega-3 PUFA ratio. Since increased intake of SFA and n-6 PUFA is considered as a cardiovascular disease risk factor, this study was conducted to determine whether a three-month dietary supplementation of high-fat diets (HFDs) with saturated fatty acids and a significant proportion of various n-6 and n-3 PUFA ratios would affect the architecture and protein expression patterns of the murine heart. Therefore, three HFD ( = 6) feeding groups: rich in SFA, dominated by PUFA with the n-6/n-3-14:1, and n-6/n-3-5:1, ratios were compared to animals fed standard mouse chow.

Urinary Proteome of Newborn Calves-New Potential in Non-Invasive Neonatal Diagnostic.

Urine is a biological diagnostic material suitable not only for the analysis of kidney and urinary tract functions but also the function of other tissues and organs. The urine proteome of adult mammals differs from the urine proteome of neonatal ones. The establishment of urinary protein maps of healthy newborn calves is important for diagnosing and monitoring the progression of various diseases.

Investigating cellular location of aquaporins in the bovine kidney. A new view on renal physiology in cattle.

To date, 13 aquaporin isoforms (AQPs) have been discovered in mammals, of which as many as 9 are located in epithelial cells lining the individual sections of the nephron and collecting tubules. Detailed analysis of the location and expression of AQPs in the kidneys of laboratory animals and humans allowed to define the key role of these proteins in renal excretion of water and other small molecules. Unfortunately, despite the significant advances in knowledge in this area, still little is known about this subject in livestock, including cattle.

Effects of an immunosuppressive treatment on the rat prostate.

The aim of this study was to determine the influence of different combinations of immunosuppressive drugs on the morphology, ultrastructure, and expression of proliferating cell nuclear antigen and cytoskeleton proteins in the rat dorsolateral prostate. The studies were conducted on 48 male Wistar rats. The animals were divided into eight groups: a control group and seven experimental groups.

Comparative study of selected blood biochemical components in milk or milk-replacer fed calves during the second week of life.

The experiment was carried out on 13 male Polish Black and White dairy calves of 75% share of the Holstein-Friesian (HF) breed during the second week of life. The animals were divided into two groups. One group (n=7) was fed mother's milk and the second (n=6) milk replacer.

Renal regulation of potassium homeostasis in calves in the first week of life including the role of atrial natriuretic peptide.

The experiment was carried out on 10 clinically healthy Polish-Friesian var. Black-and-White cow calves, during the first seven days of postnatal life. The results indicate that renal removal of potassium depends primarily on the quantity reabsorbed in the tubules, whereas clearance of the electrolyte, due to stable levels in the blood plasma, depends on the amount excreted in the urine.

Renal regulation of sodium, potassium and chloride balance in single- and twin-pregnant goats.

The aim of these studies was to analyse and compare changes in selected parameters of renal function in terms of water-electrolyte balance regulation in single- and twin-pregnant goats. Clearance analyses were carried out on 16 pregnant White Improved goats (8 in single and 8 in twin gestation). Blood plasma and urine samples were analysed for the concentration of inulin, endogenous creatinine, sodium, potassium, and chlorides.

Effect of converting enzyme inhibitor on copper and iron concentrations of blood plasma in calves during the neonatal period.

The converting enzyme catalyzes the conversion of angiotensin I to angiotensin II. Ang II is the key component of the renin-angiotensin-aldosterone (RAA) system, regulating water-electrolyte balance in newborn calves. Captopril is an inhibitor of the angiotensin-converting enzyme.

Renal regulation of calcium-phosphate metabolism in single- and twin pregnant goats.

The aim of this study was the clearance assessment of renal function in single- and twin-pregnant goats and a comparison of selected parameters associated with calcium-phosphate management in terms of litter size. Clearance studies were carried out on 16 pregnant Polish White Improved goats (8 single pregnancies and 8 twin pregnancies). It was demonstrated that the kidneys of pregnant goats regulate the calcium-phosphate balance to a great extent.