Publications by authors named "Kamil Frackowiak"
Article Synopsis
- The study focuses on the complex mechanisms of one-electron protein oxidation, investigating the reaction of sensitized photo-oxidation using carboxybenzophenone (CB) and a specific protein, MtN13.
- Researchers used crystallography and mass spectrometry to determine how CB interacts with MtN13, identifying specific amino acid residues that form adducts with the sensitizer.
- The findings reveal new mechanisms for protein oxidation, highlighting the role of ground state complexes and the influence of surrounding amino acids on the oxidation process, particularly in relation to tyrosine.
Unexpected light emission from tyrosyl radicals as a probe for tyrosine oxidation.
Free Radic Biol Med
June 2020
Tyrosine residues (Tyr) on proteins are a favoured site of one-electron oxidation due to their low one-electron reduction potentials. In this work, light-induced oxidation of Tyr residues was investigated using direct ionisation (via 266 nm light excitation) and sensitized photo-oxidation (by 3-carboxybenzophenone as sensitizer and 355 nm). Light emission (fluorescence) was observed at 410-440 nm as a result of Tyr oxidation.
View Article and Find Full Text PDF