Cleaned Meta Pseudo Labels-Based Pet Behavior Recognition Using Time-Series Sensor Data.

With the increasing number of households owning pets, the importance of sensor data for recognizing pet behavior has grown significantly. However, challenges arise due to the costs and reliability issues associated with data collection. This paper proposes a method for classifying pet behavior using cleaned meta pseudo labels to overcome these issues.

ERK-dependent IL-6 positive feedback loop mediates resistance against a combined treatment using danusertib and BKM120 in Burkitt lymphoma cell lines.

This study was conducted to define the synergistic effect of the PI3K inhibitor BKM120 with the pan-Aurora kinase inhibitor danusertib and the potential mechanism of resistance to the combined inhibitor treatment in Burkitt lymphoma cell lines. The combination of danusertib and BKM120 showed a synergistic effect on Namalwa cells but not on BJAB cells. The combined treatment led to ERK hyperactivation and induced IL-6 secretion in BJAB cells but not in Namalwa cells.

Guanosine tetra- and pentaphosphate increase antibiotic tolerance by reducing reactive oxygen species production in .

The pathogen is the causative agent of cholera. Emergence of antibiotic-resistant strains is increasing, but the underlying mechanisms remain unclear. Herein, we report that the stringent response regulator and stress alarmone guanosine tetra- and pentaphosphate ((p)ppGpp) significantly contributes to antibiotic tolerance in We found that N16961, a pandemic strain, and its isogenic (p)ppGpp-overexpressing mutant ΔΔ are both more antibiotic-resistant than (p)ppGpp (ΔΔΔ) and Δ mutants, which cannot produce or utilize (p)ppGpp, respectively.

A Genetic Screen Reveals Novel Targets to Render Sensitive to Lysozyme and Cell Wall-Targeting Antibiotics.

is capable of establishing airway infections. Human airway mucus contains a large amount of lysozyme, which hydrolyzes bacterial cell walls. , however, is known to be resistant to lysozyme.

Selective and Efficient Elimination of with a Chemical Modulator that Targets Glucose Metabolism.

, a Gram-negative bacterium, is the causative agent of pandemic cholera. Previous studies have shown that the survival of the pandemic El Tor biotype strain N16961 requires production of acetoin in a glucose-rich environment. The production of acetoin, a neutral fermentation end-product, allows to metabolize glucose without a pH drop, which is mediated by the production of organic acid.

Network-assisted investigation of virulence and antibiotic-resistance systems in Pseudomonas aeruginosa.

Pseudomonas aeruginosa is a Gram-negative bacterium of clinical significance. Although the genome of PAO1, a prototype strain of P. aeruginosa, has been extensively studied, approximately one-third of the functional genome remains unknown.

The ferrichrome receptor A as a new target for Pseudomonas aeruginosa virulence attenuation.

Pseudomonas aeruginosa is an opportunistic pathogen, known to develop robust biofilms. Its biofilm development increases when antibiotics are presented at subminimal inhibitory concentrations (MICs) for reasons that remain unclear. In order to identify genes that affect biofilm development under such a sublethal antibiotic stress condition, we screened a transposon (Tn) mutant library of PAO1, a prototype P.

Extended longevity and robust early-stage development of Caenorhabditis elegans by a soil microbe, Lysinibacillus sphaericus.

Caenorhabditis elegans, originally isolated from soil, is a nematode used in various fields of biological research including host–microbe interaction. While bacterial pathogens responsible for human infections have been actively studied in C. elegans, very few bacterial species that provide beneficial effects on C.

Cholera toxin production during anaerobic trimethylamine N-oxide respiration is mediated by stringent response in Vibrio cholerae.

As a facultative anaerobe, Vibrio cholerae can grow by anaerobic respiration. Production of cholera toxin (CT), a major virulence factor of V. cholerae, is highly promoted during anaerobic growth using trimethylamine N-oxide (TMAO) as an alternative electron acceptor.

Functional screening of a metagenomic library reveals operons responsible for enhanced intestinal colonization by gut commensal microbes.

Evidence suggests that gut microbes colonize the mammalian intestine through propagation as an adhesive microbial community. A bacterial artificial chromosome (BAC) library of murine bowel microbiota DNA in the surrogate host Escherichia coli DH10B was screened for enhanced adherence capability. Two out of 5,472 DH10B clones, 10G6 and 25G1, exhibited enhanced capabilities to adhere to inanimate surfaces in functional screens.

Activation of cholera toxin production by anaerobic respiration of trimethylamine N-oxide in Vibrio cholerae.

Vibrio cholerae is a gram-negative bacterium that causes cholera. Although the pathogenesis caused by this deadly pathogen takes place in the intestine, commonly thought to be anaerobic, anaerobiosis-induced virulence regulations are not fully elucidated. Anerobic growth of the V.

Vitamin B12-mediated restoration of defective anaerobic growth leads to reduced biofilm formation in Pseudomonas aeruginosa.

Pseudomonas aeruginosa undergoes cell elongation and forms robust biofilms during anaerobic respiratory growth using nitrate (NO(3)(-)) as an alternative electron acceptor. Understanding the mechanism of cell shape change induced upon anaerobiosis is crucial to the development of effective treatments against P. aeruginosa biofilm infection.