Three-point-supported 2-DOF large-area tilting mirror inspired by a playground facility.

In this paper, we demonstrate a three-point-supported 2D optical scanner designed to enhance the field of view (FoV) and optical scanning angles, and facilitate the development of large mirrors (>10 ). By integrating fused deposition modeling (FDM) 3D printing with electromagnetic driving, we present a cost-effective, large-sized scanning mirror with two-dimensional scanning capabilities. The mirror has an area of 144 , enabling greater light capture for enhanced scanning performance.

Cost-effective 3D-printed rotatable reflectors for two-dimensional beam steering.

In this paper, we have developed a 2D optical scanning module comprising cascaded 3D-printed one-axis rotating mirrors with large areas (30×30 for the X-direction scan and 60×25 for the Y-direction scan). Each mirror device contains a square or rectangular silicon substrate coated with aluminum, serving as the mirror. A 3D-printed structure, including the mirror frame (with four embedded mini permanent magnets on the backside), torsion springs, and base, is combined with the mirror; two electromagnets are situated under the mirror as the actuation mechanism.

High antimicrobial activity of lactoferricin-expressing Bacillus subtilis strains.

The lactoferricin expressed in Bacillus subtilis is relatively low in yield, making it hard to apply in industrial settings. We constructed a six tandem repeat of lactoferricin cDNA driven by promoter PtrnQ. After transformation, two transformants P245 and P263 possessing a stable inheritance of plasmid and high expression of lactoferricin were selected.

Using as a Host Cell to Express an Antimicrobial Peptide from the Marine Chordate .

molecule against microbes-A24 (CiMAM) isolated from the marine chordate is an antimicrobial peptide. To generate CiMAM-expressing transgenic , we constructed a plasmid expressing recombinant CiMAM (rCiMAM) and introduced it into Transgenic strains C117 and C166 were selected since they were able to highly and stably express rCiMAM. We studied the bactericidal activity of pepsin-digested extracts from rCiMAM-expressing strains against freshwater and euryhaline pathogens that commonly occur in aquaculture ponds and found no difference from that of lactoferricin-expressing strains.

Enzyme-crosslinked gene-activated matrix for the induction of mesenchymal stem cells in osteochondral tissue regeneration.

Unlabelled: The development of osteochondral tissue engineering is an important issue for the treatment of traumatic injury or aging associated joint disease. However, the different compositions and mechanical properties of cartilage and subchondral bone show the complexity of this tissue interface, making it challenging for the design and fabrication of osteochondral graft substitute. In this study, a bilayer scaffold is developed to promote the regeneration of osteochondral tissue within a single integrated construct.

Cranioplasty Using a Novel Osteoconductive Scaffold and Platelet Gel.

Background: Commonly used materials for cranioplasty include autogenous bone grafts, methyl methacrylate, and titanium mesh. We evaluated a novel osteoconductive scaffold [N-isopropylacrylamide cross-linked with acrylic acid using γ-rays (ANa powder)] mixed with platelet gel for cranioplasty.

Methods: ANa powder mixed with platelet gel was implanted into a 15 × 15-mm, full-thickness calvarial bone defect in 5 New Zealand white rabbits.

Electrothermally-Actuated Micromirrors with Bimorph Actuators--Bending-Type and Torsion-Type.

Three different electrothermally-actuated MEMS micromirrors with Cr/Au-Si bimorph actuators are proposed. The devices are fabricated with the SOIMUMPs process developed by MEMSCAP, Inc. (Durham, NC, USA).

Tailored design of electrospun composite nanofibers with staged release of multiple angiogenic growth factors for chronic wound healing.

The objective of this research study is to develop a collagen (Col) and hyaluronic acid (HA) inter-stacking nanofibrous skin equivalent substitute with the programmable release of multiple angiogenic growth factors (vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), basic fibroblast growth factor (bFGF) and endothelial growth factor (EGF)) either directly embedded in the nanofibers or encapsulated in the gelatin nanoparticles (GNs) by electrospinning technology. The delivery of EGF and bFGF in the early stage is expected to accelerate epithelialization and vasculature sprouting, while the release of PDGF and VEGF in the late stage is with the aim of inducing blood vessels maturation. The physiochemical characterizations indicate that the Col-HA-GN nanofibrous membrane possesses mechanical properties similar to human native skin.

Dental optical coherence tomography.

This review paper describes the applications of dental optical coherence tomography (OCT) in oral tissue images, caries, periodontal disease and oral cancer. The background of OCT, including basic theory, system setup, light sources, spatial resolution and system limitations, is provided. The comparisons between OCT and other clinical oral diagnostic methods are also discussed.

Treatment of nonhealing diabetic lower extremity ulcers with skin graft and autologous platelet gel: a case series.

Lower extremity ulcers in diabetic patients are difficult to treat. Recently, the use of human blood platelet-derived components in this indication has been raising interest. In this study, we have evaluated the safety and efficacy of the combination of autologous platelet gel (PG) and skin graft for treating large size recalcitrant ulcers.

Single-donor allogeneic platelet fibrin glue and osteoconductive scaffold in orbital floor fracture reconstruction.

Background: Commonly used materials for orbital floor fracture reconstruction include autologous cranial bone graft and titanium mesh. We have evaluated here a biomaterial combining biphasic calcium phosphate (hydroxyapatite [HA]/β-tricalcium phosphate [TCP]) osteoconductive scaffold with single-donor allogeneic platelet fibrin glue.

Methods: The study was conducted on 10 consecutive patients with a follow-up of up to 4 years.

Modeling of micro cat's eye retroreflectors using a matrix-based three-dimensional ray tracing technique.

In this paper we develop a three-dimensional (3D) ray tracing tool based on the ABCD ray transfer matrices. With symmetric optical components and under paraxial approximation, two sets of 2×2 ABCD matrices, each for a two-dimensional subspace, can be used to describe the 3D ray propagation completely. Compared to commercial ray-tracing software packages, our tool requires no tedious drawing, and the results for various conditions, such as different device dimensions and incident angles, can be easily obtained by simply changing the parameter values used for the calculation.

Aorta fluorescence imaging by using confocal microscopy.

The activated leukocyte attacked the vascular endothelium and the associated increase in VEcadherin number was observed in experiments. The confocal microscopic system with a prism-based wavelength filter was used for multiwavelength fluorescence measurement. Multiwavelength fluorescence imaging based on the VEcadherin within the aorta segment of a rat was achieved.

Diffuse optical multipatch technique for tissue oxygenation monitoring: clinical study in intensive care unit.

Diffuse optical multipatch technique is used to assess spatial variations in absorption and scattering in biological tissue, by monitoring changes in the concentration of oxyhemoglobin and deoxyhemoglobin. In our preliminary study, the temporal tracings of tissue oxygenation are measured using diffuse optical multipatch measurement and a venous occlusion test, employing normal subjects and ICU patients suffering from sepsis and heart failure. In experiments, obvious differences in tissue oxygenation signals were observed among all three groups.

Solar concentrator constructed with a circular prism array.

We present a novel idea to construct a solar concentrator with a circular prism array. FRED ray tracing software is used to evaluate our proposed structure in which the incident light rays are deflected by total internal reflection and the optical energy is concentrated and collected at the center. The light rays to be collected travel within the disk once they enter the module, saving the space that is reserved for ray propagation in other concentrators.

Combination of guided osteogenesis with autologous platelet-rich fibrin glue and mesenchymal stem cell for mandibular reconstruction.

Background: This study examined whether a combination of autologous platelet-rich fibrin glue (PRFG) with mesenchymal stem cells (MSCs) and MEDPOR as guided tissue regeneration (GTR) could act as an osteogenic substitute and whether this treatment yields faster new bone formation than MEDPOR alone or PRFG plus MSC.

Material: MSCs were harvested and isolated from the bone marrow of dog ilium. Full-thickness bony defects (1.

A novel technique combining platelet gel, skin graft, and fibrin glue for healing recalcitrant lower extremity ulcers.

Background: There is no ideal procedure for the treatment of chronic skin ulcers. The use of platelet gel (PG) in this indication is raising interest.

Objective: To evaluate the safety and efficacy of a new procedure combining allogeneic single-donor PG and fibrin glue (FG) to enhance skin graft take for treating recalcitrant ulcers.

Evaluation of bone growth using artificial bone substitute (osteoset) and platelet gel mixtures: a preliminarily study in dogs.

Platelet gels (PG), activated by bovine thrombin (BT), have increasingly been used in orthopedic surgery. However, BT may induce immunological reactions and carry potential viral and prion risks. To avoid these side effects, thrombin derived from human plasma (human thrombin, HT) is becoming the preferred platelet activator to prepare PG.

Cranioplasty using osteoconductive scaffold and platelet glue.

Background: An alternative to autogenous bone grafts or to methyl methacrylate in the reconstruction of full-thickness calvarial bone defect is needed.

Methods: The safety and efficacy of biphasic calcium phosphate osteoconductive scaffold (Triosite) combined with platelet glue for the reconstruction of posttraumatic calvarial bone defect was evaluated in six consecutive patients. Follow-up averaged 30 months.

Analysis of the interchannel response in a MEMS 1 x N(2) wavelength-selective switch.

A theoretical model based on Fourier optics and the power-coupling overlap integral is built to investigate the interchannel response in a micro-electro-mechanical systems 1xN(2) wavelength-selective switch. The simulation results demonstrate that the interchannel response depends significantly on the output port location and the radius of curvature of the micromirrors. For the output originally aligned with the input along the dispersion direction, it is possible to achieve interchannel-response suppression by rotating the two-dimensional (2D) collimator array by a slight angle, e.

Fibrin glue mixed with gelatin/hyaluronic acid/chondroitin-6-sulfate tri-copolymer for articular cartilage tissue engineering: the results of real-time polymerase chain reaction.

Autologous fibrin glue has been demonstrated as a potential scaffold with very good biocompatibility for neocartilage formation. However, fibrin glue has been reported not to provide enough mechanical strength, but with many growth factors to interfere the tissue growth. Gelatin/hyaluronic acid/chondroitin-6-sulfate (GHC6S) tri-copolymer sponge has been prepared as scaffold for cartilage tissue engineering and showed very good results, but problems of cell seeding and cell distribution troubled the researchers.