Identification and validation of the cellular senescence-associated molecular pattern and diagnostic markers for osteoporosis.

Background: Osteoporosis is an age-related skeletal disorder with an increasing burden of osteoporotic fractures worldwide, it is urgent the identification of reliable molecular characteristics to prevent the progression of severe osteoporosis.

Methods: Two datasets were obtained from the Gene Expression Omnibus (GEO) to identify differentially expressed genes (DEGs) and selected the cellular senescence-related genes (SRGs). Consensus clustering analysis was performed based on differentially expressed SRGs (DE-SRGs).

EP300/HIF-1 pathway: the key to sacubitril valsartan sodium alleviating myocardial hypoxia injury.

Exploring the molecular mechanism underlying the pathogenesis and treatment of ischaemic cardiomyopathy (ICM) is crucial for its precise management.In this study, an ICM cell model was constructed, treated with sacubitril/valsartan, and subjected to exogenous modulation of EP300 expression. RT-qPCR and Western blot were employed to detect the expression of E1A-associated protein P300 (EP300), Hypoxia-Inducible Factor-1 (HIF-1) α, and HIF-1β.