Single-molecule imaging reveals the role of membrane-binding motif and C-terminal domain of RNase E in its localization and diffusion in .

In , RNase E is the key enzyme for RNA processing and mRNA degradation. Despite the conserved function across bacteria, the domain composition of RNase E varies significantly among species, possibly affecting the enzyme's subcellular localization, mobility, and function. In this work, we used super-resolution microscopy to find that 93% of RNase E is localized to the membrane in and exhibits slow diffusion comparable to polysomes diffusing in the cytoplasm.