Int J Syst Evol Microbiol
July 2025
Rapid and economical DNA sequencing has resulted in a revolution in phylogenomics. The impact of changes in nomenclature can be perceived as an absolute necessity of scientific rigour, coupled with the slight inconvenience of needing to re-learn names. In relation to practical aspects of microbiology, for example, infectious disease diagnosis, there may, however, be potential dangers.
View Article and Find Full Text PDFInt J Syst Evol Microbiol
March 2025
A motile, rod-shaped and anaerobic strain WK13 was isolated from a secondary root canal infection of a human tooth. WK13 cells were Gram-stain-negative, catalase-positive and oxidase-negative. The major fatty acids (≥ 5.
View Article and Find Full Text PDFHere, we report on the one hundred and twenty-five bacterial strains made available by the National Collection of Type Cultures in 2022 alongside a commentary on the strains, their provenance and significance.
View Article and Find Full Text PDFInt J Syst Evol Microbiol
December 2023
A novel bacterial strain, GSTT-20 was isolated from an infected, prosthetic endovascular graft explanted from a shepherd in London, United Kingdom. This strain was an aerobic, catalase-positive, oxidase-negative, Gram-stain-negative, motile, curved rod. It grew on blood agar, chocolate agar and MacConkey agar incubated at 37 °C in an aerobic environment after 48 h, appearing as yellow, mucoid colonies.
View Article and Find Full Text PDFThe National Collection of Type Cultures (NCTC) was founded on 1 January 1920 in order to fulfil a recognized need for a centralized repository for bacterial and fungal strains within the UK. It is among the longest-established collections of its kind anywhere in the world and today holds approximately 6000 type and reference bacterial strains - many of medical, scientific and veterinary importance - available to academic, health, food and veterinary institutions worldwide. Recently, a collaboration between NCTC, Pacific Biosciences and the Wellcome Sanger Institute established the NCTC3000 project to long-read sequence and assemble the genomes of up to 3000 NCTC strains.
View Article and Find Full Text PDFMicrobiol Resour Announc
August 2022
Here, we report on the 47 bacterial strains made available by the National Collection of Type Cultures in 2021, alongside a commentary on these strains and their significance.
View Article and Find Full Text PDFWe take advantage of a historic collection of 133 strains accessioned between 1924 and 2016, whose genomes have been long-read sequenced as part of a major National Collection of Type Cultures (NCTC) initiative, to conduct a gene family-wide computational analysis of enterotoxin genes. We identify two novel staphylococcal enterotoxin (pseudo)genes ( and ), the former of which has not been observed in any contemporary strain to date. We provide further information on five additional enterotoxin genes or gene variants that either have recently entered the literature or for which the nomenclature or description is currently unclear (, , , , and ).
View Article and Find Full Text PDFMicrobiol Resour Announc
October 2019
is a Gram-positive coccus found in the environment and within normal human skin microbiota, and more recently, it has been potentially implicated as an opportunistic pathogen. Here, we describe the genome sequences of five strains of (NCTC2676, NCTC7514, NCTC7512, NCTC7528, and NCTC7511).
View Article and Find Full Text PDFThe sixth global cholera pandemic lasted from 1899 to 1923. However, despite widespread fear of the disease and of its negative effects on troop morale, very few soldiers in the British Expeditionary Forces contracted cholera between 1914 and 1918. Here, we have revived and sequenced the genome of NCTC 30, a 102-year-old Vibrio cholerae isolate, which we believe is the oldest publicly available live V.
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