Ligand multimerization effects on binding efficiency of Protein L affinity chromatography resins.

Nearly four decades have passed since the discovery of protein-L. Its exceptional ability in binding to the kappa light chain of immunoglobulins makes it a suitable candidate for the purification of certain biotherapeutics, particularly antibody fragments. Efforts have focused on improving its recovery and dynamic binding capacity.

Simultaneous detection of bovine and porcine DNA in pharmaceutical gelatin capsules by duplex PCR assay for Halal authentication.

Background: In the pharmaceutical industry, hard- and soft-shelled capsules are typically made from gelatin, commonly derived from bovine and porcine sources. To ensure that pharmaceutical products comply with halal regulations in Muslim countries (no porcine products allowed), development of a valid, reliable, quick, and most importantly, cost-effective tests are of utmost importance.

Methods: We developed a species-specific duplex polymerase chain reaction (PCR) assay targeting 149 bp porcine and 271 bp bovine mitochondrial DNA (mtDNA) to simultaneously detect both porcine and bovine DNA (in one reaction at the same time) in gelatin.