Isolation of Elasnin and Antimycin Derivatives from Syo_1.56 SARP-Expressing sp. RK18-A0406 and Their Biological Activities.

Exploration of pathway-specific regulators is an important strategy to uncover hidden natural products. Recently, we successfully enhanced the production of novel elasnins by expressing Syo_1.56 antibiotic regulatory protein (SARP) in sp.

Expression of Syo_1.56 SARP Regulator Unveils Potent Elasnin Derivatives with Antibacterial Activity.

Actinomycetes are prolific producers of natural products, particularly antibiotics. However, a significant proportion of its biosynthetic gene clusters (BGCs) remain silent under typical laboratory conditions. This limits the effectiveness of conventional isolation methods for the discovery of novel natural products.

Isolation of new lucilactaene derivatives from P450 monooxygenase and aldehyde dehydrogenase knockout Fusarium sp. RK97-94 strains and their biological activities.

Fusarium sp. RK97-94 is a producer of potent antimalarial compounds such as lucilactaene and its derivatives. The biosynthetic gene cluster of lucilactaene was identified but only a knockout mutant of methyltransferase (luc1) was reported in previous papers.

Dihydrolucilactaene, a Potent Antimalarial Compound from sp. RK97-94.

A recently discovered secondary metabolism regulator, NPD938, was used to alter the secondary metabolite profile in sp. RK97-94. Three lucilactaene analogues were detected via UPLC-ESI-MS analysis in NPD938-treated culture.