Brook trout (Salvelinus fontinalis) is a socioeconomically important fish species for fisheries, aquaculture, and aquatic conservation. We produced a 2.5-Gb reference assembly by combining Hi-C chromosome conformation capture with high-coverage short- and long-read sequencing of a fully homozygous mitotic gynogenic doubled-haploid fish, which facilitates the assembly of highly complex salmonid genomes.
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