Combined effects of hypoxia and thermal stress on hemocyte response in Pacific oysters (Crassostrea gigas): Insights from transcriptomic and proteomic analyses.

Hypoxia and elevated seawater temperatures are increasingly prevalent stressors in marine ecosystems, significantly impacting the physiology of marine organisms. This study investigates the transcriptomic and proteomic responses of Pacific oyster (Crassostrea gigas) hemocytes to hypoxia alone (water temperature, 23 °C; dissolved oxygen [DO] level, 1 mg O₂/L) and combined hypoxia with high temperature (water temperature, 28 °C; DO level, 1 mg O₂/L) over a 10-day exposure period. Using RNA sequencing and liquid chromatography-mass spectrometry, we identified distinct molecular responses to these stressors.

Evaluation of the diagnostic reliability of the gill-based Ray's fluid thioglycollate medium assay for Perkinsus olseni infections in Manila clams (Ruditapes philippinarum) across Korean tidal flats.

The alveolate protozoan parasite Perkinsus olseni infects a range of marine bivalves inhabiting tidal flats and shallow subtidal zones, causing considerable damage to shellfish industries. Infection by P. olseni is typically assessed using Ray's fluid thioglycollate medium (RFTM) assay, with gill tissue often employed as a diagnostic proxy for whole-body infection.

Insights into the Genetic Connectivity and Climate-Driven Northward Range Expansion of (Gastropoda: Turbinidae) Along the Eastern Coast of Korea.

, a commercially and ecologically significant marine gastropod traditionally found in Jeju Island and the southern coast of Korea, is experiencing a reported northward expansion into the East Sea, likely influenced by rising seawater temperatures. This study provides preliminary genetic insights into the genetic structure and connectivity of populations between Jeju and the East Sea using mitochondrial COI sequences. Samples from 6 geographically distinct locations were analyzed, with three cloned replicates generated to enhance sequence reliability.

EnhanceCenter for improving point based tracking and rich feature representation.

In this study, we propose EnhanceCenter, a multiple-object tracking model that demonstrates enhanced tracking efficiency and stability while reducing dependencies on computationally intensive detectors. EnhanceCenter, based on the CenterTrack method, introduces three key improvements. First, a channel-spatial-spatial feature fusion module effectively utilizes object appearance information, enhancing tracking in complex scenes.

Effect of diet changes in benthic ecosystems owing to climate change on the physiological responses of Turbo sazae in waters around Jeju Island, Korea.

The benthic ecosystem in temperate regions is becoming barren owing to the replacement of macroalgae by calcareous algae as a result of climate change-induced increases in water temperature. The aim of this study was to observe how the top shell, Turbo sazae, an invertebrate that feeds on macroalgae, adapts to a benthic environment with a reduced macroalgae population owing to replacement by calcareous algae. Using tank experiments, the top shells were cultured for 18 weeks.

Sublethal impacts of Hebei Spirit oil spill on the growth and reproductive physiology of the Pacific oyster Crassostrea gigas at Taean on the West Coast of Korea (2008-2010).

Following the Hebei Spirit oil spill (HSOS) in December 2007, benthic organisms of the Taean coast on the west coast of Korea were heavily affected. A month after HSOS, the alkyl PAHs in the oyster body elevated to 13,500 ng/g dry wt, although it dropped to 5335 ng/g dry wt in December 2008, and returned to the levels before the accident (547-858 ng/g dry wt) by 2010. In 2008 spring, the damaged oysters exhibited deteriorated growth and reproduction, exhibiting signs of physiological stress.

In vitro impact of Bisphenol A on the immune functions of primary cultured hemocytes of Pacific abalone (Haliotis discus hannai).

This study investigated the toxic effects of Bisphenol A (BPA) on the Pacific abalone (Haliotis discus hannai) using in vitro assays with primary cultured hemocytes. The abalone hemocytes were exposed to BPA concentrations up to 100 μM to assess cytotoxicity. Subsequently, hemocytes were exposed to sublethal BPA concentrations (LC = 2.

First report of Perkinsus honshuensis in the variegated carpet shell clam Ruditapes variegatus in Korea.

The recent discovery of Perkinsus honshuensis, a new Perkinsus species infecting Manila clams Ruditapes philippinarum (Sowerby, 1852), in Japan, suggested that, based on proximity, P. honshuensis could also be in Korean waters, where to date, P. olseni was believed to be the only Perkinsus species present.

Isolation and identification of Perkinsus olseni from feces and marine sediment using immunological and molecular techniques.

Molecular and immunological probes were used to identify various life stages of Perkinsus olseni, a protozoan parasite of the Manila clam Ruditapes philippinarum, from a marine environment and decomposing clam tissue. Western blotting revealed that the antigenic determinants of the rabbit anti-P. olseni antibody developed in this study were peptides with molecular masses of 55.

Effects of peroxisome proliferator-activated receptor-gamma 2 Pro12Ala polymorphism on body fat distribution in female Korean subjects.

The effects of peroxisome proliferator-activated receptor gamma 2 (PPAR gamma 2) Pro12Ala (P12A) polymorphism on body mass index (BMI) and type 2 diabetes are well documented; however, until now, only a few studies have evaluated the effects of this polymorphism on body fat distribution. This study was conducted to elucidate the effects of this polymorphism on computed tomography (CT)-measured body fat distribution and other obesity-related parameters in Korean female subjects. The frequencies of PPAR gamma 2 genotypes were: PP type, 93.

The effects of uncoupling protein-1 genotype on lipoprotein cholesterol level in Korean obese subjects.

Uncoupling protein-1 (UCP-1) plays a major role in thermogenesis, and has been implicated in the pathogenesis of obesity and metabolic disorders. The purpose of this study was to estimate the effects of A-3826G polymorphism of the UCP-1 gene on the plasma lipid profiles in 190 Korean obese subjects with a body mass index (BMI) more than 30 kg/m2. Height, weight, BMI, wait-to-hip ratio (WHR), obesity index, and body composition were measured and genotype of UCP-1 was analyzed by polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) method.