Induction of Nephron-Ductal Dual Lineages via Early Retinoic Acid Signaling Establishes a Platform for Fully Patterned Kidney Organoids.

Kidney organoids are powerful tools for renal disease modeling and nephrotoxicity screening, yet their limited structural complexity-particularly the underdevelopment of ureteric bud (UB) lineages-remains a major limitation. A novel differentiation protocol is developed that short-term activation of retinoic acid (RA) signaling during the early intermediate mesoderm (IM) stage, enabling co-induction of anterior and posterior IM lineages. This eliminates the need for UB co-culture and supports the formation of kidney organoids containing complete nephron segments.

Prebiotic potential and metabolic benefits of rhizome-derived polysaccharides in a high-fat diet-induced obesity model.

Metabolic dysfunction-associated steatotic liver disease (MASLD) is a global health issue that causes severe liver abnormalities and metabolic dysregulation, incurring substantial costs to healthcare systems. , a traditional remedy, has been studied for its anti-inflammatory and neuroprotective effects. However, its potential role in the management of metabolic disorders via its polysaccharide components remains unclear.

Preventive role of Pastinaca sativa in mitigating metabolic dysfunction-associated steatotic liver disease via modulation of metabolic endotoxemia.

Metabolic dysfunction-associated steatotic liver disease (MASLD) is a leading global liver disorder. Parsnip (Pastinaca sativa), rich in dietary fiber and medicinal properties, has shown health benefits, but its effects on MASLD are largely unexplored. This study evaluated the prebiotic and anti-MASLD effects of parsnip root water-soluble extract (PRE) in a mouse model.

Prebiotic Activity of Rhizome Extract and Its Effects on Obesity and Gut Inflammation.

(sweet flag), a medicinal plant, especially its rhizome, shows strong antioxidant and anti-inflammatory effects; however, its efficacy in treating intestinal inflammation and obesity is still unexplored. In this study, we investigated the prebiotic activity of sweet flag rhizome extract (SRE) and its preventive effects against high-fat diet (HFD)-induced obesity and colonic inflammation. The prebiotic activity was assessed based on the prebiotic activity scores of four probiotic strains.

Lactococcus lactis KF140 Ameliorates Nonalcoholic Fatty Liver Disease Induced by N-Carboxymethyl-Lysine and High-Fat Diet.

Scope: Long-term consumption of excessive dietary advanced glycation end-products such as N-carboxymethyl-lysine (CML), which are produced by the Maillard reaction during food thermal processing, leads to nonalcoholic fatty liver disease (NAFLD) along with high fat consumption. The study previously finds that administration of Lactococcus lactis KF140 (LL-KF140) detoxifies CML by decreasing CML absorption both in a rat model and clinical trial.

Methods And Results: The present study evaluates the ameliorative effect of LL-KF140 on NAFLD and fatty liver-related biomarkers in a mouse model induced by CML and high fat.

Ameliorating effects of Orostachys japonica against high-fat diet-induced obesity and gut dysbiosis.

Ethnopharmacological Relevance: Orostachys japonica (rock pine) has been used as a folk remedy to treat inflammation, hepatitis, and cancer in East Asia.

Aim Of The Study: The aim of this study was to investigate the effect of rock pine extract (RPE) on high-fat diet-induced obesity in mice and to examine its effects on gut dysbiosis.

Materials And Methods: The characteristic compound of RPE, kaempferol-3-O-rutinoside, was quantified using high-performance liquid chromatography.

Resistant starch-enriched brown rice exhibits prebiotic properties and enhances gut health in obese mice.

Resistant starch serves as a prebiotic in the large intestine, aiding in the maintenance of a healthy intestinal environment and mitigating associated chronic illnesses. This study aimed to investigate the impact of resistant starch-enriched brown rice (RBR) on intestinal health and functionality. We assessed changes in resistant starch concentration, structural alterations, and branch chain length distribution throughout the digestion process using an in vitro model.

Thyme Extract Alleviates High-Fat Diet-Induced Obesity and Gut Dysfunction.

Prolonged intake of a high-fat diet (HFD) disturbs the composition of gut microbiota, contributing to the development of metabolic diseases, notably obesity and increased intestinal permeability. Thyme ( L.), an aromatic plant, is known for its several therapeutic properties.

Dietary N-(carboxymethyl)lysine is a trigger of non-alcoholic fatty liver disease under high-fat consumption.

The irreversible glycation of proteins produces advanced glycation end products (AGEs) which are triggered to bind the receptor for AGE (RAGE), thereby activating mitogen-activated protein kinase/nuclear factor-κB signaling pathway and stimulating proinflammatory cytokines, ultimately leading to chronic disorders. In this study, we focus the promoting effect of N-carboxymethyl-lysine (CML), one of the most dietary AGEs, on non-alcoholic fatty liver disease (NAFLD) and evaluated NAFLD-related biomarkers. Oxidative stress and hepatic steatosis were assessed in oleic acid (OA)-induced HepG2 cells.

Structural characteristics of resistant starch-enriched rice during digestion and its effects on gut barrier function in high-fat induced obese mice.

Dietary supplementation with indigestible carbohydrates is known to improve the gut environment and prevent obesity and inflammatory diseases by modulating the gut microbiota. In previous work, we established a method for the production of resistant starch (RS)-enriched high-amylose rice (R-HAR) using citric acid. The present study aimed to evaluate changes in structural characteristics during digestion of R-HAR and its effects on the gut health.

Anti-obesity effects of red pepper ( L.) leaf extract on 3T3-L1 preadipocytes and high fat diet-fed mice.

Patients with obesity mostly have metabolic syndrome and this can lead to multiple health problems. In the present study, we evaluated the anti-obesity effect of water-soluble red pepper ( L.) leaf extract (PLE) on 3T3-L1 adipocytes and high-fat diet (HFD)-fed mice.

Gellan gum prevents non-alcoholic fatty liver disease by modulating the gut microbiota and metabolites.

Gellan gum (GG) is an anionic polysaccharide used as an additive in the food industry. However, the effect of GG on gut microbiota regulation and nonalcoholic fatty liver disease (NAFLD) has not yet been investigated. In vitro fermentation experiments have demonstrated that GG promoted the growth of probiotic strains such as Lactiplantibacillus rhamnosus and Bifidobacterium bifidum, producing metabolites beneficial to gut health.

KF140 Reduces Dietary Absorption of N - (Carboxymethyl)lysine in Rats and Humans β-Galactosidase Activity.

Background And Aims: Excessive intake of advanced glycation end products (AGEs), which are formed in foods cooked at high temperatures for long periods of time, has negative health effects, such as inflammatory responses and oxidative stress. N-(Carboxymethyl)lysine (CML) is one of the major dietary AGEs. Given their generally recognized as safe status and probiotic functionalities, lactic acid bacteria may be ideal supplements for blocking intestinal absorption of food toxicants.

ssp. MG741 Reduces Body Weight and Ameliorates Nonalcoholic Fatty Liver Disease via Improving the Gut Permeability and Amelioration of Inflammatory Cytokines.

Diet-induced obesity is one of the major causes of the development of metabolic disorders such as insulin resistance and nonalcoholic fatty liver disease (NAFLD). Recently, specific probiotic strains have been found to improve the symptoms of NAFLD. We examined the effects of ssp.

Pectic polysaccharides: Targeting gut microbiota in obesity and intestinal health.

The gut microbiome is a complex ecosystem of the host body that maintains a balance with its host. In this context, dysbiosis can lead to inflammatory response, immune dysregulation, and various metabolic disorders. Dietary polysaccharides mediate gut microbiota and its metabolites related to host health.

Dietary rhamnogalacturonan-Ⅰ rich extracts of molokhia ameliorate high fat diet-induced obesity and gut dysbiosis.

Obesity is a global health issue associated with increased prevalence of disease and mortality. Molokhia (Corchorus olitorius L.) leaves, used as vegetables in Asia and Africa, comprise abundant water-soluble mucilage polysaccharides.

Amelioration of Hepatic Steatosis in Mice through CBA7346-Mediated Regulation of High-Fat Diet-Induced Insulin Resistance and Lipogenesis.

Dietary habits and gut microbiota play an essential role in non-alcoholic fatty liver disease (NAFLD) and related factors such as insulin resistance and de novo lipogenesis. In this study, we investigated the protective effects of CBA7346, isolated from the gut of healthy Koreans, on mice with high-fat diet (HFD)-induced NAFLD. Administration of CBA7346 reduced body and liver weight gain, serum alanine aminotransferase and aspartate aminotransferase levels, liver steatosis, and liver triglyceride levels in mice on an HFD; the strain also decreased homeostatic model assessment for insulin resistance values, as well as serum cholesterol, triglyceride, lipopolysaccharide, leptin, and adiponectin levels in mice on an HFD.

Polysaccharide fraction from greens of Raphanus sativus alleviates high fat diet-induced obesity.

Radish (Raphanus sativus) greens are commonly used as a vegetable in Korea; however, their anti-obesity effect has not been reported yet. We prepared the polysaccharide fraction of radish greens (PRG) and assessed its anti-obesity activity in high fat diet (HFD)-induced obese C57BL/6J mice. Supplementation with 4 mg/kg PRG reduced weight gain and body fat percentage, and regulated serum biomarkers against HFD-induced obesity.

Molokhia leaf extract prevents gut inflammation and obesity.

Ethnopharmacological Relevance: Molokhia is highly consumed in Egypt as edible and medicinal plants, and its leaves are used for the treatment of pain, fever, and inflammation.

Aim Of The Study: High-fat diet (HFD) induces gut dysbiosis, which is closely linked to metabolic diseases including obesity and leaky gut. The effects of molokhia (Corchorus olitorius L.

Dietary Carbohydrate Constituents Related to Gut Dysbiosis and Health.

Recent studies report that microbiota in the human intestine play an important role in host health and that both long- and short-term diets influence gut microbiota. These findings have fueled interest in the potential of food to promote health by shaping the intestinal microbiota. Despite the fact that large populations in Asia consume high quantities of carbohydrates, such diets have been ignored in comparison to the attention received by Western diets containing high quantities of fat and animal protein.