()-edited petunia shows reduced seed germination, seed set, seed vigor, and growth.

Unlabelled: In this study, we evaluated the impact of editing the powdery mildew-susceptible gene in cv. 'Mirage Rose' using CRISPR/Cas9. We evaluated plant growth, seed physiology, pollen viability and germination, and gibberellin (GA)-associated gene expression.

Production of genetically stable and Odontoglossum ringspot virus-free Cymbidium orchid 'New True' plants via meristem-derived protocorm-like body (PLB) subcultures.

Background: This study aimed to produce Odontoglossum ringspot virus (ORSV)-free Cymbidium orchid 'New True' plants from ORSV-infected mother plants by culturing their meristems and successively repeating subcultures of protocorm-like bodies (PLBs) derived from the meristems.

Results: Initially, ORSV was confirmed as the causative agent of viral symptoms in orchid leaves via reverse transcription-polymerase chain reaction (RT-PCR) analysis. Meristems from infected plants were cultured to generate PLBs, which in sequence were repeatedly subcultured up to four times.