Ultra-performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry-Based Profiling and Evaluation of Antioxidant, Antityrosinase, Antimicrobial and Antiproliferative Activities of Eulophia nuda Lindl.: A Medicinal Orchid.

Eulophia nuda Lndl., a terrestrial medicinal orchid, is well known for its ethnomedicinal applications in treating bronchitis, tuberculosis, tumours, snake bites and arthritis. This study aimed to identify the phytoconstituents of E.

Mixed Trabecular and Mucinous Carcinoid Arising in the Background of a Mature Ovarian Cystic Teratoma.

Ovarian carcinoid tumors, originating from mature ovarian cystic teratomas (dermoid cysts), are rare neuroendocrine tumors. The synchronous occurrence of trabecular and mucinous carcinoid tumors within a single ovarian teratoma is infrequent and has limited documentation. We present the case of a 67-year-old postmenopausal woman presenting with abdominal pain, constipation, and urinary retention, but without features of carcinoid syndrome.

Association of Red Cell Distribution Width and Mean Platelet Volume With Disease Activity in Rheumatoid Arthritis Patients.

Background: The inner layer of the synovial joints is the primary target of rheumatoid arthritis, or RA, a chronic systemic inflammatory disorder that is linked to increasing disability, early mortality, and economic hardships. The objective is to determine the association of red cell distribution width (RDW) and mean platelet volume (MPV) with disease activity in RA.

Material And Methods: A retrospective study was conducted between July 2021 and January 2022 in the outpatient rheumatology clinics at Gulab Devi Teaching Hospital.

Fault Classification for Cooling System of Hydraulic Machinery Using AI.

Hydraulic systems are used in all kinds of industries. Mills, manufacturing, robotics, and Ports require the use of Hydraulic Equipment. Many industries prefer to use hydraulic systems due to their numerous advantages over electrical and mechanical systems.

Role of Ondansetron in Reducing Methotrexate Intolerance in Patients with Inflammatory Arthritis.

Objective: To determine the frequency of intolerance to Methotrexate (MTX) in patients with inflammatory arthritis by using MTX intolerance severity score, and evaluate the effects of Ondansetron in reducing MTX intolerance.

Study Design: Interventional study.

Place And Duration Of Study: Rheumatology clinic, Combined Military Hospital (CMH), Lahore, from 1st November 2021 to 30th April 2022.

Profiles of Whole Blood Transfusion Recipients at a Teaching Hospital in Lahore, Pakistan.

Background Blood transfusion plays a vital role in medical practice. Evaluation of blood utilization in a blood bank is a crucial step in good transfusion practice. It is the inception towards assessing the present and future demands for blood and also avoiding unnecessary transfusions.

Expression of p53 Protein in Prostate Carcinoma and its Correlation with Gleason Grade.

Objectives: To determine the correlation of p53 expression with Gleason score in prostate cancer.

Study Design: Descriptive study.

Place And Duration Of Study: Department of Pathology, Fatima Memorial Hospital, Lahore; from January 2014 to August 2016.

uPAR antibody (huATN-658) and Zometa reduce breast cancer growth and skeletal lesions.

Urokinase plasminogen activator receptor (uPAR) is implicated in tumor growth and metastasis due to its ability to activate latent growth factors, proteases, and different oncogenic signaling pathways upon binding to different ligands. Elevated uPAR expression is correlated with the increased aggressiveness of cancer cells, which led to its credentialing as an attractive diagnostic and therapeutic target in advanced solid cancer. Here, we examine the antitumor effects of a humanized anti-uPAR antibody (huATN-658) alone and in combination with the approved bisphosphonate Zometa (Zoledronic acid) on skeletal lesion through a series of studies in vitro and in vivo.

DNA methylation signatures of breast cancer in peripheral T-cells.

Background: Immune surveillance acts as a defense mechanism in cancer, and its disruption is involved in cancer progression. DNA methylation reflects the phenotypic identity of cells and recent data suggested that DNA methylation profiles of T cells and peripheral blood mononuclear cells (PBMC) are altered in cancer progression.

Methods: We enrolled 19 females with stage 1 and 2, nine with stage 3 and 4 and 9 age matched healthy women.

Methyl donor S-adenosylmethionine (SAM) supplementation attenuates breast cancer growth, invasion, and metastasis ; therapeutic and chemopreventive applications.

DNA hypomethylation coordinately targets various signaling pathways involved in tumor growth and metastasis. At present, there are no approved therapeutic modalities that target hypomethylation. In this regard, we examined the therapeutic plausibility of using universal methyl group donor S-adenosylmethionine (SAM) to block breast cancer development, growth, and metastasis through a series of studies using two different human breast cancer cell lines (MDA-MB-231 and Hs578T) and using an MDA-MB-231 xenograft model of breast cancer.

Presentation of parathyroid adenoma with genu valgum and thoracic deformities.

Parathyroid adenoma is the main cause of primary hyperparathyroidism. It is usually asymptomatic and occurs more commonly in adults. It presents with raised parathormone (PTH) and Ca+ levels in serum.

A common promoter hypomethylation signature in invasive breast, liver and prostate cancer cell lines reveals novel targets involved in cancer invasiveness.

Unlabelled: Cancer invasion and metastasis is the most morbid aspect of cancer and is governed by different cellular mechanisms than those driving the deregulated growth of tumors. We addressed here the question of whether a common DNA methylation signature of invasion exists in cancer cells from different origins that differentiates invasive from non-invasive cells. We identified a common DNA methylation signature consisting of hyper- and hypomethylation and determined the overlap of differences in DNA methylation with differences in mRNA expression using expression array analyses.

S-adenosylmethionine blocks osteosarcoma cells proliferation and invasion in vitro and tumor metastasis in vivo: therapeutic and diagnostic clinical applications.

Osteosarcoma (OS) is an aggressive and highly metastatic form of primary bone cancer affecting young children and adults. Previous studies have shown that hypomethylation of critical genes is driving metastasis. Here, we examine whether hypermethylation treatment can block OS growth and pulmonary metastasis.

Genome-wide study of hypomethylated and induced genes in patients with liver cancer unravels novel anticancer targets.

Purpose: We utilized whole-genome mapping of promoters that are activated by DNA hypomethylation in hepatocellular carcinoma (HCC) clinical samples to shortlist novel targets for anticancer therapeutics. We provide a proof of principle of this approach by testing six genes short-listed in our screen for their essential role in cancer growth and invasiveness.

Experimental Design: We used siRNA- or shRNA-mediated depletion to determine whether inhibition of these genes would reduce human tumor xenograft growth in mice as well as cell viability, anchorage-independent growth, invasive capacities, and state of activity of nodal signaling pathways in liver, breast, and bladder cancer cell lines.

Meningioma like tumour of thyroid: a rare variant of follicular adenoma.

Spindle cell lesions of thyroid are uncommon. Meningioma like tumour of thyroid is a rare variant of follicular adenoma, which can easily be misdiagnosed. One such case is being reported here with detailed histological, histochemical and immunohistochemical findings.

Association of raised intraocular pressure and its correlation to the energy used with raised versus normal intraocular pressure following Nd: YAG laser posterior capsulotomy in pseudophakes.

Objective: To determine the frequency of raised intraocular pressure (IOP) after Nd: YAG laser posterior capsulotomy and its association with the energy used with raised versus normal intraocular pressure in pseudophakes.

Study Design: Comparative, cross-sectional study.

Place And Duration Of Study: Ophthalmology Department, PNS Shifa Hospital, Karachi, from August 2008 to February 2009.

Central mucoepidermoid carcinoma, a case report with molecular analysis of the TORC1/MAML2 gene fusion.

Mucoepidermoid carcinoma is the most common salivary gland malignancy. The majorities of these tumors arise in the parotid and minor salivary glands, but may rarely develop intraosseously. The latter is not uncommonly associated with diagnostic and management difficulties.