Changes in ruminoreticular temperature and body activity in pregnant Hanwoo cows () after lumpy skin disease vaccination.

The first outbreak of lumpy skin disease (LSD) occurred in South Korea in October 2023, and cattle are being vaccinated countrywide to prevent its spread. However, studies regarding the changes in body temperature and activity after LSD vaccination during pregnancy are lacking. Therefore, this study aimed to compare the ruminoreticular temperature and body activity of 18 pregnant and 28 non-pregnant cows using a bolus sensor after LSD vaccination.

MSTN knockout enhances the production of MYOD1-mediated steak-type cultivated meat.

Background: As the global population increases, the demand for protein sources is expected to increase, driving the demand for cell-based cultivated meat. This study aimed to enhance the productivity of cultivated meat through optimization of the cell source and organization process.

Results: We engineered fibroblasts into myogenic cells via non-viral introduction of the MYOD1 gene, avoiding viral methods for safety.

Cas9-expressing cattle using the PiggyBac transposon all-in-one system.

Background: Livestock, particularly cattle, are crucial for biotechnology fields, such as genetic breeding, infectious diseases, bioreactors, and specific disease models. However, genetic engineering in cattle has lagged due to long gestation periods, single embryo pregnancies, and high rearing costs. Additionally, the slow validation of germline transmission and the absence of germline-competent embryonic stem cells hinder progress.

Mammalian ubiquitous promoter isolated from proximal regulatory region of bovine MSTN gene.

Gene therapy is a promising method for treating inherited diseases by directly delivering the correct genetic material into patient cells. However, the limited packaging capacity of vectors poses a challenge. Minimizing promoter size is a viable strategy among various approaches to address this issue.

Long-term (10-year) monitoring of transposon-mediated transgenic cattle.

The production of transgenic animals using non-viral methods has raised questions regarding their long-term health and genomic stability. In this study, we evaluated these aspects in transgenic cattle over ten years, using transposon-mediated gene transfer. Our longitudinal analysis included a comprehensive health assessment and whole-genome DNA resequencing.

Stable long-term germline transmission of GFP transgenic rat via PiggyBac transposon mediated gene transfer.

Transgene silencing provides a significant challenge in animal model production via gene engineering using viral vectors or transposons. Selecting an appropriate strategy, contingent upon the species is crucial to circumvent transgene silencing, necessitating long-term observation of in vivo gene expression. This study employed the PiggyBac transposon to create a GFP rat model to address transgene silencing in rats.

Outlook on genome editing application to cattle.

In livestock industry, there is growing interest in methods to increase the production efficiency of livestock to address food shortages, given the increasing global population. With the advancements in gene engineering technology, it is a valuable tool and has been intensively utilized in research specifically focused on human disease. In historically, this technology has been used with livestock to create human disease models or to produce recombinant proteins from their byproducts.

Generation of double knockout cattle via CRISPR-Cas9 ribonucleoprotein (RNP) electroporation.

Background: Genome editing has been considered as powerful tool in agricultural fields. However, genome editing progress in cattle has not been fast as in other mammal species, for some disadvantages including long gestational periods, single pregnancy, and high raising cost. Furthermore, technically demanding methods such as microinjection and somatic cell nuclear transfer (SCNT) are needed for gene editing in cattle.

Supplement of secreted recombinant low molecular weight human fibroblast growth factor 2 in culture media enhances in vitro bovine maturation.

With the annual increase in in vitro bovine embryo production, understanding oocyte maturation is becoming more important. Previous studies have shown that oocyte maturation can be improved by adding bovine additives to in vitro maturation media. Among the additives, human fibroblast growth factor 2 (hFGF2) is well known for its positive influence on the growth rate and quality of cells and oocytes.

Germline transmission of MSTN knockout cattle via CRISPR-Cas9.

Although the production of several founder animals (F0) for gene editing in livestock has been reported in cattle, very few studies have assessed germline transmission to the next generation due to the long sexual maturation and gestation periods. The present study aimed to assess the germline transmission of MSTN mutations (-12bps deletion) in MSTN mutant F0 male and female cattle. For this purpose, oocytes and semen were collected after the sexual maturation of MSTN cattle, and embryos produced by in vitro fertilization were analyzed.

Change of Ruminoreticular Temperature and Body Activity before and after Parturition in Hanwoo () Cows.

How do body temperature and activity change before and after parturition in pregnant cows? Changes in body temperature such as ruminal, rectal, and vaginal temperature during the parturition have been reported, but there are no results of the simultaneous observation of body temperature and activity. The aim of this study was to simultaneously confirm changes in the ruminoreticular temperature and body activity before and after parturition using the ruminoreticular bio-capsule sensor every 1 h. The 55 pregnant cows were used for the experiment, the ruminoreticular bio-capsule sensor was inserted and stabilized, and the ruminoreticular temperature and body activity were measured.

Production of MSTN-mutated cattle without exogenous gene integration using CRISPR-Cas9.

Many genome-edited animals have been produced using clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 technology to edit specific genes. However, there are few guidelines for the application of this technique to cattle. The goal of this study was to produce trait-improved cattle using the genome-editing technology CRISPR-Cas9.