Flow Cytometric Immunophenotyping of Mixed Phenotype Acute Leukemia in a Tertiary Care Hospital of Eastern Odisha.

 Acute lymphoblastic leukemia affects both adults and children. Mixed phenotype acute leukemia (MPAL) is a rare subset featuring blasts with multiple lineage-specific antigens. Diagnosis is made via flow cytometric immunophenotyping with specific CD markers.

Vitamin D Deficiency is Associated With Dengue Severity in Pediatric Cases, Eastern India.

Vitamin D is an important modulator of host immune response during immunity development in context to several diseases in children. This study included 280 pediatric dengue cases, which were further stratified as dengue fever (DF), dengue with warning signs (DWS), and severe dengue (SD), wherein naturally circulating vitamin D metabolite, 25(OH)D was assessed, followed by statistical analysis for elucidating the role of serum 25(OH)D in dengue severity. qRT-PCR based serotyping showed that dengue virus (DENV)-2 was the major circulating DENV serotype in the patients.

Simultaneous detection of dengue virus serotypes in a dual-serotype-detection nucleic acid based lateral flow assay.

Dengue virus (DENV) is an important arthropod-borne viral disease, with four antigenically and genetically diverse serotypes (DENV-1, DENV-2, DENV-3, and DENV-4). Timely and accurate diagnosis of dengue virus serotypes is crucial for the management of outbreaks. This study focussed on the development of a RT-PCR based lateral flow strip assay to detect DENV serotypes in a dual detection manner without using gel electrophoresis.

RT-PCR Result of SARS-CoV-2 Viral RNA in Cadavers and Viral Transmission Risk to Handlers.

Unlabelled: During the onset of the pandemic, a common research question was asked by the hospital staff, and family members who were handling COVID-19-infected cadavers, "does COVID-19-positive dead body harbor SARS-CoV-2 viral RNA?" Several research findings were reported but due to the lack of proper research findings, the question remained unanswered. The present study was planned to observe the virus transmission risk from cadavers to the handlers. A pilot study was conducted on 54 cadavers who died in COVID-ICU (SARS-CoV-2-positive diagnosed by RT-PCR) during 2021-2022.

Role of endoplasmic reticulum stress-related unfolded protein response and its implications in dengue virus infection for biomarker development.

Dengue virus (DENV) causes debilitating disease in humans, which varies at different rates in host cells, such as monocytes, macrophages, dendritic cells, Langerhans cells, and other cell types. Such heterogeneity in DENV infection in cells could be attributed to a range of factors, including host cell immune response, anti-viral cellular proteins, and virus mediated cellular autophagy. This review delineates an important feature of every cell, the unfolded protein response (UPR) that is attributed to the accumulation of several viral and unfolded/misfolded proteins, such as in DENV infection.

A Rare Case of Left-Sided Gastroschisis in a Human Museum Specimen.

Gastroschisis is a congenital defect in the anterior abdominal wall resulting in herniation of the abdominal viscera without any fetal membrane covering it. It usually occurs to the right of a normally inserted umbilical cord. The anomaly is associated with intrauterine growth retardation, stillbirth, and preterm delivery.